基于G-四链体电化学发光生物传感器灵敏检测端粒酶活性OA北大核心CSTPCD
Sensitive Detection of Telomerase Activity Based on a G-quadru-plex Electrochemiluminescent Biosensor
端粒酶活性的分析检测对于癌症诊断、预后治疗等具有重要意义.该研究利用G-四链体(G4)DNA的金属配合物靶向发光探针[Ir(ppy)2(pip)]PF6,构建了灵敏检测癌细胞端粒酶活性的电化学发光(ECL)生物传感器.首先将引物DNA组装在电极表面,然后与癌细胞提取的端粒酶共同孵育,具有活性的端粒酶在引物末端生成端粒重复序列从而形成G4结构,随后[Ir(ppy)2(pip)]PF6通过与G4靶向作用结合到延长的序列中.结果表明[Ir(ppy)2(…查看全部>>
The analysis and detection of telomerase activity is important for cancer diagnosis,prog-nosis,and treatment.In this study,an electrochemiluminescence(ECL)biosensor was constructed for sensitive detection of telomerase activity in cancer cells using a metal complex-targeted lumines-cent probe[Ir(ppy)2(pip)]PF6 for G-quadruplex(G4)DNA.Primer DNA was first assembled on the electrode surface and then co-incubated with telomerase extracted from cancer cells.The …查看全部>>
贺彰瑾;杜雨莹;鲁理平
北京工业大学 环境科学系,北京 100124北京工业大学 环境科学系,北京 100124北京工业大学 环境科学系,北京 100124
化学
端粒酶活性G-四链体金属配合物电化学发光生物传感器
telomerase activityG-quadruplexmetal complexelectrochemiluminescencebio-sensor
《分析测试学报》 2024 (7)
963-970,8
国家自然科学基金资助项目(22276005,21876005)
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