基于G-四链体电化学发光生物传感器灵敏检测端粒酶活性OA北大核心CSTPCD

The analysis and detection of telomerase activity is important for cancer diagnosis,prog-nosis,and treatment.In this study,an electrochemiluminescence(ECL)biosensor was constructed for sensitive detection of telomerase activity in cancer cells using a metal complex-targeted lumines-cent probe[Ir(ppy)2(pip)]PF6 for G-quadruplex(G4)DNA.Primer DNA was first assembled on the electrode surface and then co-incubated with telomerase extracted from cancer cells.The active telomerase generated telomeric repeats at the end of the primer to form the G4 structure,and then[Ir(ppy)2(pip)]PF6 bound to the extended sequence by targeting with G4.The results showed that the ECL signal of[Ir(ppy)2(pip)]PF6 showed a positive correlation with telomerase activity,and the ECL intensity of the sensor showed a good linear correlation with the concentration of cancer cells in the range of 10-5×105 cell/mL,with the limit of detection(LOD)of 3 cell/mL.The experiments also demonstrated that the sensor had good stability,applicability,anti-interference ablity,and repro-ducibility.Finally,the sensor was used for the determination of telomerase activity in real human se-rum and urine samples,and satisfactory results were also obtained.