Py/GC-MS法检测安全套润滑剂遗留微量物证OA北大核心CSTPCD

In this study,a highly sensitive detection method for trace evidence of condom lubricants was established using Py/GC-MS.Simulated sexual assault case samples were prepared,extracted using hexane ultrasonication,processed through membrane filtration,and evaporated under N2 flow to 50 μL.The collected extract was transferred to a pyrolysis crucible using a pipette,evaporated sol-vent completely,and analyzed by Py/GC-MS.Methodological investigations showed that the quanti-tative limit of this method was 5 μg/kg,with recoveries of 91.0％to 103％and relative standard devia-tions(RSDs)of 7.1％to 8.3％.The method was used to analyze PDMS standards and simulated sam-ples made from the top 20 best-selling condoms from online sellers,assessing interference from bio-logical fluids(blood,urine,saliva)and the persistence on different media(underwear,skin).PD-MS was detected in all silicone-based lubricated condom samples,excluding interference from blank samples.Biological fluids did not interfere with PDMS detection,and the longest attachment times in underwear and skin environments were 72 hours and 48 hours,respectively,establishing a detection time window after sexual assault incidents.This method provides an accurate and reliable way to de-tect trace evidence of condom lubricants.