超高效液相色谱-四极杆-飞行时间高分辨质谱用于化妆品中15种前列腺素类似物的快速筛查和测定OA北大核心CSTPCD

In this study,an ultra performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry(UPLC-Q-TOF MS)method was developed for the rapid screening and quantitative analysis of 15 prostaglandin analogues in cosmetics.The analytes in sample were dis-persed in saturated sodium chloride solution(wax samples were dispersed with tetrahydrofuran),then extracted with acetonitrile.After centrifugation and filtration,the UPLC separation of 15 prosta-glandin analogues was performed on Poroshell 120 EC-C18 column(150 mm×3.0 mm,2.7 μm),us-ing 5 mmol/L ammonium formate(containing 0.1％formic acid)solution and acetonitrile as mobile phase by gradient elution.15 prostaglandin analogues were detected by positive ion electrospray ion-ization(ESI)in the Targeted MS/MS mode.To construct a screening library with the software by the retention time,exact mass of primary parent ions,isotope abundance ratio and secondary sub-ion li-brary matching were collected.The results of matching were used to rapid identification of 15 prosta-glandin analogues in cosmetics.The quantification of primary parent ions was performed by external standard method.15 prostaglandin analogues were all well separated.The linear correlation coeffi-cients(r2)were more than 0.99.The limits of detection(LODs)ranged of 0.3-17.6 μg/g and the lim-its of quantitation(LOQs)ranged of 1.1-58.8 μg/g.The recoveries at three spiked levels in lotion,cream,emulsion,powder and gel matrices varied from 70.1％to 134％,with relative standard devi-ations not more than 7.5％.The established method is rapid,simple and accurate,which is suitable for the screening and determination of 15 prostaglandin analogues in cosmetics,providing technical support for cosmetic risk identification.