分析测试学报2024，Vol.43Issue(7)：1011-1017,7.DOI:10.12452/j.fxcsxb.24033102
超高效液相色谱-四极杆-飞行时间高分辨质谱用于化妆品中15种前列腺素类似物的快速筛查和测定
Rapid Screening and Determination of 15 Prostaglandin Analogues in Cosmetics by Ultra Performance Liquid Chromatography-Quadru-pole-Time-of-flight High-resolution Mass Spectrometry
摘要
Abstract
In this study,an ultra performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry(UPLC-Q-TOF MS)method was developed for the rapid screening and quantitative analysis of 15 prostaglandin analogues in cosmetics.The analytes in sample were dis-persed in saturated sodium chloride solution(wax samples were dispersed with tetrahydrofuran),then extracted with acetonitrile.After centrifugation and filtration,the UPLC separation of 15 prosta-glandin analogues was performed on Poroshell 120 EC-C18 column(150 mm×3.0 mm,2.7 μm),us-ing 5 mmol/L ammonium formate(containing 0.1％formic acid)solution and acetonitrile as mobile phase by gradient elution.15 prostaglandin analogues were detected by positive ion electrospray ion-ization(ESI)in the Targeted MS/MS mode.To construct a screening library with the software by the retention time,exact mass of primary parent ions,isotope abundance ratio and secondary sub-ion li-brary matching were collected.The results of matching were used to rapid identification of 15 prosta-glandin analogues in cosmetics.The quantification of primary parent ions was performed by external standard method.15 prostaglandin analogues were all well separated.The linear correlation coeffi-cients(r2)were more than 0.99.The limits of detection(LODs)ranged of 0.3-17.6 μg/g and the lim-its of quantitation(LOQs)ranged of 1.1-58.8 μg/g.The recoveries at three spiked levels in lotion,cream,emulsion,powder and gel matrices varied from 70.1％to 134％,with relative standard devi-ations not more than 7.5％.The established method is rapid,simple and accurate,which is suitable for the screening and determination of 15 prostaglandin analogues in cosmetics,providing technical support for cosmetic risk identification.关键词
超高效液相色谱-四极杆-飞行时间高分辨质谱/快速筛查/化妆品/前列腺素类似物Key words
ultra performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry/rapid screening/cosmetics/prostaglandin analogues分类
化学化工引用本文复制引用
陈安丽,张静,黄子沣,严小红..超高效液相色谱-四极杆-飞行时间高分辨质谱用于化妆品中15种前列腺素类似物的快速筛查和测定[J].分析测试学报,2024,43(7):1011-1017,7.基金项目
广州市市场监督管理局科技项目(2022kj28) （2022kj28）
