高效液相色谱法测定鸡蛋中25种咪唑类兽药及其代谢物残留OA北大核心CSTPCD

The method for the determination of 25 imidazole veterinary drugs and their metabolites in eggs was established by high performance liquid chromatography(HPLC).The egg solution was ex-tracted and concentrated by acetonitrile,purified by Oasis PRIME HLB solid phase extraction col-umn.The samples were eluted by gradient with methanol and water as mobile phases,separated by ZORBAX SB-Aq(250 mm×4.6 mm,5 μm)column,detected by diode array detector(DAD),and quantified by external standard method.The results demonstrated that the 25 compounds showed good linear relationships in their linear ranges with the correlation coefficients(r2)greater than 0.999.And the limits of detection(LODs)and quantification(LOQs)were 0.8-5.0 p.g/kg and 2.5-12.5 μg/kg,respectively.The average recoveries ranged from 78.5％to 103％and the relative stan-dard deviations(RSDs)ranged from 1.1％to 6.5％at three concentration levels.The method has no obvious matrix effect with high accuracy and good precision,which can be used for the daily detec-tion of 25 imidazole veterinary drugs and metabolites residues in eggs.