分析测试学报2024，Vol.43Issue(7)：1018-1024,7.DOI:10.12452/j.fxcsxb.24032705
高效液相色谱法测定鸡蛋中25种咪唑类兽药及其代谢物残留
Determination of 25 Imidazole Veterinary Drugs and Their Metabo-lites in Eggs by High Performance Liquid Chromatography
摘要
Abstract
The method for the determination of 25 imidazole veterinary drugs and their metabolites in eggs was established by high performance liquid chromatography(HPLC).The egg solution was ex-tracted and concentrated by acetonitrile,purified by Oasis PRIME HLB solid phase extraction col-umn.The samples were eluted by gradient with methanol and water as mobile phases,separated by ZORBAX SB-Aq(250 mm×4.6 mm,5 μm)column,detected by diode array detector(DAD),and quantified by external standard method.The results demonstrated that the 25 compounds showed good linear relationships in their linear ranges with the correlation coefficients(r2)greater than 0.999.And the limits of detection(LODs)and quantification(LOQs)were 0.8-5.0 p.g/kg and 2.5-12.5 μg/kg,respectively.The average recoveries ranged from 78.5％to 103％and the relative stan-dard deviations(RSDs)ranged from 1.1％to 6.5％at three concentration levels.The method has no obvious matrix effect with high accuracy and good precision,which can be used for the daily detec-tion of 25 imidazole veterinary drugs and metabolites residues in eggs.关键词
苯并咪唑/硝基咪唑/代谢物/高效液相色谱法/鸡蛋Key words
benzimidazole/nitroimidazole/metabolite/high performance liquid chromatography/egg分类
化学化工引用本文复制引用
邱月,余秋玲,谭超兰,陆嘉莉,杨倩..高效液相色谱法测定鸡蛋中25种咪唑类兽药及其代谢物残留[J].分析测试学报,2024,43(7):1018-1024,7.基金项目
重庆市科技局科研院所绩效激励引导专项(cstc2022jxjl50001) （cstc2022jxjl50001）
国家市场监督管理总局科技计划项目(2023MK106) （2023MK106）
重庆市市场监督管理局科研计划项目(CQSJKJ2022040,CQSJKJDW2023003) （CQSJKJ2022040,CQSJKJDW2023003）
