高等学校化学学报2024，Vol.45Issue(7)：1-17,17.DOI:10.7503/cjcu20240139
核酸等温扩增技术在病毒检测中的应用
Development of Nucleic Acid Isothermal Amplification Technologies for Virus Detection
摘要
Abstract
Viruses play a significant role in causing human diseases,and traditional PCR techniques have been widely used for their molecular diagnosis.However,the temperature requirements of PCR limit its application in field diagnostics.To address the need for rapid on-site diagnosis,isothermal nucleic acid amplification technologies have emerged as a promising alternative.These technologies enable nucleic acid amplification at a constant temperature without the need for thermal cycling,making them more adaptable for different diagnostic settings.This comprehen-sive review examines the latest advancements in isothermal amplification technologies for virus detection.It covers various aspects,including viral sample collection,nucleic acid extraction,and isothermal amplification detection.The review explores the principles,key parameters,and applications of enzyme-assisted isothermal amplification,enzyme-free isothermal amplification,and cascade amplification techniques integrated with multiple systems.Furthermore,a comparison of commercially available reagent kits is provided to highlight their respective characteristics.Additionally,the review discusses the current challenges faced by isothermal nucleic acid amplification technologies in pathogen detection,such as extraction efficiency,stability,and cost,and proposes future directions to enhance the on-site diagnostic efficacy of these technologies.关键词
等温扩增技术/CRISPR检测/核酸扩增/环境病毒/病毒提取Key words
Isothermal amplification technology/CRISPR assay/Nucleic acid amplification/Environmental viruses/Virus detection分类
生物学引用本文复制引用
肖航,王小燕,邓兆佳,廖文静,谢文菁,彭汉勇..核酸等温扩增技术在病毒检测中的应用[J].高等学校化学学报,2024,45(7):1-17,17.基金项目
国家自然科学基金(批准号:22276199)、国家重点研发计划项目(批准号:2022YFC3701302,2023YFA0915102)和中国科学院先导专项(批准号:XDB0750100)资助.Supported by the National Natural Science Foundation of China(No.22276199),the National Key Research and Development Program of China(Nos.2022YFC3701302,2023YFA0915102)and the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB0750100). （批准号:22276199）
