广西植物2024，Vol.44Issue(6)：1017-1027,11.DOI:10.11931/guihaia.gxzw202309012
藏药黄帚橐吾化学成分及抗炎活性研究
Chemical constituents and anti-inflammatory activity from Ligularia virgaurea
摘要
Abstract
Ligularia virgaurea is one of the original plants of the Tibetan medicine"Rixiao"for the treatment of clearing heat and removing yellow water.In order to study the chemical constituents and anti-inflammatory activity of L.virgaurea,the compounds were separated by silica gel,Sephadex LH-20 gel,ODS gel column chromatography and other column chromatography technologies.The structures of all isolates were identified by spectroscopic methods(NMR and HR-ESI-MS).Their inhibitory activity of the compounds on nitric oxide(NO)was determined by lipopolysaccharide(LPS)-induced RAW264.7 cell model.The results were as follows:(1)Twenty-one compounds were separated and identified from petroleum ether and n-butanol extracts of L.virgaurea,including spiroeuryolide(1),cacalol acetate(2),oplopenone(3),8-ethyl-palmosalide A(4),1-hydroxy-3,7-dimethyl-2-(pent-3-enyl)benzofuran(5),syringaresinol-O-β-D-glucopyranoside(6),pinoresinol-O-β-D-glucopyranoside(7),isoeucommin A(8),eucommin A(9),6,7-dimethoxycoumarin(10),ferulic acid(11),ethyl caffeate(12),methyl caffeate(13),methyl ferulate(14),ethyl ferulate(15),caffeic acid(16),2-[(2′E)-3′,7′-dimethyl-2′,6′-octadienyl]-4-methoxy-6-methylphenol(17),2,8-dimethyl-6-methoxy-2-(4′-methylpent-3′-enyl)-chromene(18),β-sitosterol(19),dodecyl(Z)-9-hexadecenoate(20)and hexacosanal(21).Compounds 1-4,6,11-16,18,20,21 were isolated from the whole herbs of L.virgaurea for the first time.(2)The anti-inflammatory activity in vitro showed that compounds 1-3,6,11-16,17,19 could significantly inhibited releases of NO at concentration ranging from 1.56 to 50.00 μmol·L-1(P<0.05 or P<0.01),Compound 5 had no inhibitory effect on the release of NO at a concentration of 50.00 μmol·L-1,but it could inhibit releases of NO at concentrations of 12.50,25.00 μmol·L-1(P<0.05).This finding enriches the chemical constituent and biological activity research of L.virgaurea and provides a certain theoretical reference for the future development and utilization of its anti-inflammatory activity.关键词
黄帚橐吾/倍半萜/化学成分/结构鉴定/抗炎活性Key words
Ligularia virgaurea/sesquiterpenes/chemical constituent/structural identification/anti-inflammatory activity分类
生物学引用本文复制引用
张馨予,罗日措,王洪玲,梁文娟..藏药黄帚橐吾化学成分及抗炎活性研究[J].广西植物,2024,44(6):1017-1027,11.基金项目
国家重点研发计划项目(2019YFC1712300) （2019YFC1712300）
国家自然科学基金(31660098) （31660098）
江西省教育厅科技项目(GJJ201215). （GJJ201215）
