广西植物2024，Vol.44Issue(6)：1082-1090,9.DOI:10.11931/guihaia.gxzw202304020
蛇含委陵菜的木脂素类成分及其细胞毒活性研究
Lignans from Potentilla kleiniana and their cytotoxicity
摘要
Abstract
Potentilla kleiniana belongs to the family Rosaceae,which distributes in Central Asia,East Asia and Southeast Asia.In China,this plant is mainly found in east,south and southwest provinces.P.kleiniana has been prescribed for the treatment of various diseases in the field of traditional Chinese medicine,such as cough,fever,tuberculosis,mastitis,rheumatoid arthritis.Our previous study found that P.kleiniana had a certain cytotoxicity on tumor cells.The purpose of this paper was to investigate the chemical constituents of P.kleiniana and their cytotoxicity on tumor cells.The 60%ethanol extract of P.kleiniana were isolated by D-101 macroporous adsorptive resins,silica gel,Sephadex LH-20,Toyopearl HW-40F,semi-preparative high performance liquid chromatography and other methods,and their chemical structures were elucidated on the basis of physicochemical properties,NMR and HR-ESI-MS analysis.Meanwhile,all these compounds were evaluated for cytotoxicity against human cervical cancer cell line Hela.The results were as follows:(1)Thirteen lignans were isolated and identified as(+)-pionresinol(1),(+)-8-hydroxypinoresinol(2),(+)-syringaresinol(3),(+)-medioresinol(4),(+)-pionresinol-4-O-β-D-glucopyranoside(5),(+)-8′-hydroxypinoresinol-4-O-β-D-glucopyranoside(6),(+)-8′-hydroxypinoresinol-4′-O-β-D-glucopyranoside(7),(+)-pinoresinol-8′-O-β-D-glucopyranoside(8),schilignan F(9),(+)-pionresinol-4,4′-O-bisglucopyranoside(10),(+)-lariciresinol-4′-O-β-D-glucopyranoside(11),neoolivil-4-O-β-D-glucopyranoside(12),3,3′-bis[3,4-dihydro-4-hydroxy-6-methoxy-2H-1-benzopyran](13).Among them,compounds 1-4,7,8,10,12,13 were isolated from genus Potentilla for the first time,and compounds 5,6,9,11 were isolated from P.kleiniana for the first time.(2)Cytotoxicity studies showed that compounds 1,3 and 4 display certain inhibitory activities against Hela cells with IC50 values of(69.94±1.89),(66.25±2.11),(59.81±1.73)μmol·L-1,respectively.Therefore,the study enriches the chemical constituents of P.kleiniana,and provides a material basis for the development of anti-cervical cancer drugs.关键词
蛇含委陵菜/化学成分/分离纯化/结构鉴定/木脂素/细胞毒性/抗肿瘤活性Key words
Potentilla kleiniana/chemical constituents/isolation and purification/structural identification/lignans/cytotoxicity/antitumor activity分类
农业科技引用本文复制引用
张宝,杨红,匡维米,陈婷婷,金倩倩,李勇军,李悦..蛇含委陵菜的木脂素类成分及其细胞毒活性研究[J].广西植物,2024,44(6):1082-1090,9.基金项目
贵州省中医药管理局中医药、民族医药科学技术研究课题项目(QZYY-2021-176) （QZYY-2021-176）
贵阳市卫生健康局高层次创新型青年卫生人才培养计划项目([2022]筑卫健科技合同字第010号). （[2022]筑卫健科技合同字第010号）
