广西植物2024，Vol.44Issue(6)：1105-1117,13.DOI:10.11931/guihaia.gxzw202307014
蛇足石杉COBRA基因家族的分子生物信息学及表达分析
Molecular bioinformatics and expression analysis of the COBRA gene family in Huperzia serrata
摘要
Abstract
In order to clarify the molecular bioinformatics characteristics and tissue expression patterns of the COBRA gene family members of Huperzia serrata,the physicochemical properties,domains,conserved motifs,cis-acting elements,and genes expression of the family members(HsCOBRAs)were analyzed by bioinformatics techniques,based on the full-length transcriptome data of the H.serrata.The results were as follows:(1)A total of 24 HsCOBRAs family members were screened in the full-length transcriptome of H.serrata,including 9 acidic proteins,11 stabilizing proteins,5 hydrophobic proteins,7 proteins with transmembrane structures,and 3 proteins with signal peptides.(2)Subcellular localization was found in the cell wall,chloroplast,nucleus,and cell membrane.(3)Structural analysis revealed that HsCOBRAs had 7 domains and 6 conserved motifs,and partial members had a highly conserved CCVS structure.(4)HsCOBRAs had 45 cis-acting elements such as CAAT-box and TATA-box.(5)HsCOBRA2 had the highest expressions in leaves,spores,stems and gemma.The study results can provide theoretical basis for further research and biological function verification of HsCOBRAs.关键词
蛇足石杉/COBRA基因家族/生物信息学/全长转录组/表达分析Key words
Huperzia serrata/COBRA gene family/bioinformatics/full-length transcriptome/expression analysis分类
生物学引用本文复制引用
黄玉妹,滕建北,涂冬萍,梁柳观..蛇足石杉COBRA基因家族的分子生物信息学及表达分析[J].广西植物,2024,44(6):1105-1117,13.基金项目
广西自然科学基金(2022JJA140897) （2022JJA140897）
广西中医药大学桂派中医药传承创新团队项目(2022B005) （2022B005）
广西中医药大学桂派杏林青年英才项目(2022C033) （2022C033）
广西中医药大学研究生教育创新计划项目(YCSY2022007). （YCSY2022007）
