广西植物2024，Vol.44Issue(6)：1159-1169,11.DOI:10.11931/guihaia.gxzw202303035
宽叶金粟兰的化学成分及其抗肿瘤活性研究
Chemical constituents from Chloranthus henryi and their antitumor activities in vitro
摘要
Abstract
In order to study the chemical constituents from Chloranthus henryi and their antitumor activities in vitro.The chemical constituents of ethyl acetate fraction,extracted with 95%ethanol from C.henryi were isolated and purified by silica gel column chromatography,reversed phase column chromatography,Sephadex LH-20 column chromatography and preparation liquid chromatography,and their structures were identified by the physicochemical properties,spectral data combined with relevant literatures.The cytotoxic activities of these compounds were evaluated by MTT method.The results were as follows:(1)Twelve compounds were isolated and identified as pipercyclobutanamide C(1),chololactone A(2),sarcanolide B(3),oxacol A(4),chloramultiol D(5),chlorasessilifol B(6),chlorajaponol(7),tianmushanol(8),spicachlorantins B(9),spicachlorantins A(10),serrachlorin A(11),chloramultiols A(12).Among them,Compound 1 was identified as a new compound,and except for Compound 8,all the other compounds were isolated from the C.henryi for the first time.(2)The cytotoxic activity test results showed that only compounds 2 and 7 had good inhibitory effects on HeLa cells in vitro,while the other compounds showed no inhibition effects.The IC50 of the two compounds were(4.50±0.27)μmol·L-1 and(4.25±0.08)μmol·L-1 respectively.In conclusion,the study enriches the chemical constituents of C.henryi,and provides a reference for the further exploration and utilization of this Chloranthus herb plants.关键词
宽叶金粟兰/化学成分/分离纯化/结构鉴定/细胞毒活性/抑制作用Key words
Chloranthus henryi/chemical constituents/isolation and purification/structural identification/cytotoxic activity/inhibitory effects分类
生物学引用本文复制引用
张卫青,朱成光,梁伟,晏晨..宽叶金粟兰的化学成分及其抗肿瘤活性研究[J].广西植物,2024,44(6):1159-1169,11.基金项目
贵州省科技计划项目(黔科合支撑[2022]一般189). （黔科合支撑[2022]一般189）
