广西植物2024，Vol.44Issue(6)：1170-1181,12.DOI:10.11931/guihaia.gxzw202302010
不同成熟度老鹰茶中酚类化合物含量及抗氧化活性研究
Contents and antioxidant activities of phenolic compounds in Hawk tea with different maturity levels
摘要
Abstract
To study the difference in the contents of phenolics and the antioxidant activities between two kinds of Hawk tea of different maturity levels,and to evaluate their qualities,the contents of 15 phenolic compounds were determined using LC-MS/MS method.Then,the antioxidant activities of two types of tea were evaluated using DPPH radical scavenging rate,ABTS+radical scavenging rate,and Fe3+ferric reducing antioxidant power(FRAP).And then,the contents of 15 phenolic compounds and the antioxidant activities between two kinds of Hawk tea of different maturity levels were analyzed by analysis of variance(ANOVA).Based on the contents of 15 phenolic compounds,hierarchical cluster analysis(HCA),principal component analysis(PCA),and orthogonal partial least square-discriminant analysis(OPLS-DA)were used to classify two kinds of Hawk tea.Finally,the contribution of different phenolic compounds in Hawk tea to antioxidation was discussed by partial least square regression analysis(PLSR).The results were as follows:(1)The contents of catechin,p-coumaric acid,isoquercitrin,hyperoside,nicotiflorin,astragalin,kaempferol,quercetin,and afzelin in tender leaf tea were significantly higher than those in old leaf tea,among which the average contents of catechin,isoquercitrin,and astragalin were higher than those in old leaf tea by 1 039.43,169.12 and 257.35 mg·100 g-1,respectively.The results of HCA,PCA,and OPLS-DA could distinguish two kinds of Hawk tea.(2)The ANOVA showed significant differences in DPPH radical scavenging rate,ABTS+radical scavenging rate,and FRAP between two kinds of tea,with the tender leaf tea superior to the old leaf tea.(3)The PLSR suggested that isoquercitrin,catechin,astragalin,chlorogenic acid,hyperoside,p-coumaric acid,and kaempferol were the main phenolic compounds those contributed to the antioxidant activity of Hawk tea.This study can provide a reference for Hawk tea's quality control and application promotion.关键词
老鹰茶/酚类化合物/LC-MS/MS/抗氧化活性/相关分析Key words
Hawk tea/phenolic compounds/LC-MS/MS/antioxidant activity/correlation analysis分类
生物学引用本文复制引用
李志荣,邵起菊,吴其妹,李影,李文阳,方灿灿,肖世基,陈荣祥..不同成熟度老鹰茶中酚类化合物含量及抗氧化活性研究[J].广西植物,2024,44(6):1170-1181,12.基金项目
国家自然科学基金(31560102,81760652) （31560102,81760652）
遵义医科大学大学生创新创业项目(ZYDC2021152,ZYDC2022035). （ZYDC2021152,ZYDC2022035）
