基础医学与临床2024，Vol.44Issue(7)：931-939,9.DOI:10.16352/j.issn.1001-6325.2024.07.0931
KAT8通过增强METTL3表达促进结直肠癌细胞系增殖
KAT8 promotes the proliferation of colorectal cancer cell lines by enhancing METTL3 expression
摘要
Abstract
Objective To investigate the role and mechanism of lysine acetyltransferase 8(KAT8)on the prolifera-tion of colorectal cancer cells.Methods The expression of KAT8 in cancerous tissues and adjacent tissues of color-ectal cancer patients was analyzed by RNA-seq data of TCGA database.Cell proliferation was detected by colony-forming unit assays and CCK8.The GEO database was used to analyze the differential genes of KAT8 knockdown cells and control cells and perform functional pathway enrichment analysis.In the colorectal cancer database hosted on cBioPortal,that conducted an analysis examining the correlation between KAT8 and genes in the regulation of N6-methyladenosine(m6A)modification.Western blot technique was employed to assess the protein expression lev-els of KAT8 and METTL3.Results Compared to human normal colorectal tissue,KAT8 was highly expressed in colorectal cancer(P＜0.05).Knockdown or selective inactivation of KAT8 inhibited colorectal cancer cells prolifera-tion(P＜0.05).In colorectal cancer cell lines,knocking down KAT8 reduced m6A modification levels(P＜0.05).Knocking down KAT8 inhibited METTL3 expression(P＜0.05).Over-expression of METTL3 reversed cell prolifera-tion which was inhibited by knockdown KAT8(P＜0.05).Conclusions KAT8 facilitates the proliferation of color-ectal cancer cell lines through regulation of METTL3-mediated m6A modifications.关键词
KAT8/METTL3/N6-甲基腺苷/细胞增殖Key words
KAT8/METTL3/N6-methyladenosion/cell proliferation分类
临床医学引用本文复制引用
张鹏举,李杰,张梦迪,孙少科,许寅喆..KAT8通过增强METTL3表达促进结直肠癌细胞系增殖[J].基础医学与临床,2024,44(7):931-939,9.基金项目
国家自然科学基金(81800552) （81800552）
军队医学科技青年培育计划-孵化项目(21QNPY108) （21QNPY108）
中央高校基本科研业务费(3332023150) （3332023150）
