基础医学与临床2024，Vol.44Issue(7)：940-946,7.DOI:10.16352/j.issn.1001-6325.2024.07.0940
丙二酸二钠通过抑制琥珀酸脱氢酶活性损伤人精子运动功能
Disodium malonate impairs human sperm motility by inhibiting succinate dehydrogenase activity
摘要
Abstract
Objective To investigate the impact of succinate dehydrogenase(SDH)on the modulation of human sperm functions.Methods The isolated human sperm were co-incubated with different concentrations(10,20,40 mmol/L)of SDH inhibitor disodium malonate for one or two hours.The activity of the SDH was measured by commercially available reagent kit,while the protein level of the SDH catalytic subunit SDHA was determined through Western blot analysis.Sperm functions were analyzed:1)The impact of disodium malonate on important mo-tility parameters of un-capcitated sperm including progressive motility rate(PR),total motility(TM),average pathvelocity(VAP)and the ability of capacitated sperm to penetrate viscous media were be assessed using a com-puter aided semen analysis system.2)Effect of disodium malonate on sperm survival rate was evaluated using the Eosin-Nigrosin microscopy.3)The incidence of acrosome reaction in capacitated sperm was be detected by PSA-FITC staining assay following disodium malonate treatment.Results Disodium malonate had no effect on expression of SDH catalytic subunit SHDA protein in human sperm.However,it inhibited the catalytic activity of the SDH,sperm forward motility,total motility,and the ability of sperm to penetrate viscous media.These inhibitory effects were positively correlated with the concentration of disodium malonate.Furthermore,disodium malonate had no any influence on the occurrence of spontaneous acrosome reaction in capacitated sperm.Conclusions Disodium mal-onate impairs human sperm motility by inhibiting succinate dehydrogenase activity.关键词
琥珀酸脱氢酶/丙二酸二钠/精子功能/精子运动Key words
succinate dehydrogenase/disodium malonate/sperm function/sperm motility分类
药学引用本文复制引用
彭真,闻琴,卢京,涂泽梁,程一民..丙二酸二钠通过抑制琥珀酸脱氢酶活性损伤人精子运动功能[J].基础医学与临床,2024,44(7):940-946,7.基金项目
国家自然科学基金(82101690) （82101690）
江西省自然科学基金(20224BAB216029) （20224BAB216029）
江西省教育厅科技项目(GJJ2201749) （GJJ2201749）
宜春市指导性科技计划项目(2023ZDJH2699) （2023ZDJH2699）
