基础医学与临床2024，Vol.44Issue(7)：989-996,8.DOI:10.16352/j.issn.1001-6325.2024.07.0989
重组人乳铁蛋白通过促进成骨细胞增殖加速大鼠骨折愈合
Recombinant human lactoferrin accelerates fracture healing in rats by promoting osteoblast proliferation
摘要
Abstract
Objective To investigate the effect of recombinant human lactoferrin(rhLF)on fracture healing in rat models with femur fracture and the proliferation of rat primary osteoblasts.Methods Male rats were randomly di-vided into 3 groups:simple fracture group(SF group),collagen film-treated group(CF group)and rhLF-com-pound collagen lamination film-treated group(CLF group).The fracture healing was observed by X-ray image and histological microscopy with HE and Masson staining.The newborn SD rats with osteoblasts were treated with differ-ent concentrations of rhLF,and osteoblast proliferation was detected by MTT method.Results The bone scabs in the CLF group were significantly larger than those in the SF and CF groups at 1,2,and 3 weeks postoperatively(P＜0.05).There was no difference in the comparison between the groups at 4th week.The proliferation of cartilag-inous and sclerotic bone scabs in the CLF group was more pronounced and showed the appearance of mature trabec-ulae and lamellar bone with a high degree of maturation of the bone scabs.The groups with 100 mg/L and 500 mg/L rhLF showed a significant decrease in A value for rat primary osteoblasts cultured in vitro as compared with the control group(P＜0.05).The A value increased significantly at the first day(P＜0.05).The concentration of rhLF increased at the fifth and tenth days.Conclusions rhLF has an accelerating effect on fracture healing which is manifested by fast scab formation,large volume and high maturity.The rhLF can promote the proliferation of os-teoblasts cultured in vitro,and the effect is mainly manifested in the rapid proliferation stage of osteoblasts.关键词
重组人乳铁蛋白/骨折愈合/成骨细胞/股骨骨折/大鼠Key words
recombinant human lactoferrin/fracture healing/osteoblast/femur fracture/rat分类
临床医学引用本文复制引用
叶俊武,郑徐洲,费林聪,李漱阳,杨天府..重组人乳铁蛋白通过促进成骨细胞增殖加速大鼠骨折愈合[J].基础医学与临床,2024,44(7):989-996,8.基金项目
西南医科大学附属医院博士启动基金(22028) （22028）
