解放军医学院学报2024，Vol.45Issue(6)：624-630,7.DOI:10.12435/j.issn.2095-5227.2024.060
三维垂体基板类器官的构建和鉴定
Construction and identification of three-dimensional pituitary placode organoids
摘要
Abstract
Background The three-dimensional pituitary placode organoids hold extensive potential applications,yet current research in this field remains limited.Objective To induce human induced pluripotent stem cells(hiPSCs)into pituitary placode organoids within an in vitro three-dimensional environment.Methods hiPSCs were cultivated in suspension to form spheroids and gradually differentiated into pituitary placode organoids through supplement with a series of cell factors,agonists,or inhibitors.qPCR was employed to measure the mRNA expression levels of NANOG,SIX1,PITX1,and LHX3 in the organoids.Immunofluorescence staining was used to detect the expression of PITX1 and LHX3 in the organoids.Results hiPSCs formed spheroids in vitro through suspension culture.As the culture time extended,the shape of the organoids changed from regular spherical to irregular.qPCR and immunofluorescence staining revealed successful expression of the pituitary placode markers PITX1 and LHX3 in the organoids at 30 and 40 days,respectively,indicating the successful construction of pituitary placode organoids.Conclusion This study preliminarily establishes pituitary placode organoids,providing a laboratory-based model for pituitary development research,drug development,and regenerative medicine therapies.关键词
诱导多能干细胞/垂体/类器官/聚合酶链反应/荧光抗体技术Key words
induced pluripotent stem cells/pituitary/organoids/polymerase chain reaction/fluorescent antibody technique分类
医药卫生引用本文复制引用
王胜杰,姜得悦,秦巧臻,江小霞,郭清华..三维垂体基板类器官的构建和鉴定[J].解放军医学院学报,2024,45(6):624-630,7.基金项目
国家自然科学基金面上项目(82270824) （82270824）
北京自然科学基金面上项目(7232153) （7232153）
