SOX4通过靶向调节miR-17表达水平对结直肠癌细胞免疫逃逸及细胞迁移的影响OACSTPCD

Objective To investigate the effect of sex determining region Y-box 4(SOX4)on immune es-cape and cell migration of colorectal cancer cells by targeting microrna(miR)-17 expression.Methods The expression levels of SOX4 and miR-17 in normal colorectal mucosal epithelial cells and 4 types of colorectal cancer cells were detected by real-time fluorescence quantitative polymerase chain reaction.Take the colorectal cancer cell was divided into colorectal cancer cell(CC)+SOX4-NC group,the colorectal cancer cells(SN),colorectal cancer cells+SOX4 agonist(SM),colorectal cancer cells+SOX4 inhibitors(SI),colorectal canc-er cell+miR-17-NC(MN)group,colorectal cancer cells+miR-17 agonist(MM)group,colorectal cancer cells+miR-17 inhibitor(MI)group,colorectal cancer cells+SOX4 inhibitor+miR-17 inhibitor(Ⅱ)group.The expression levels of programmed death-1(PD-1)and PD-1 ligand 1(PD-L1)were detected by Western blot.The number of cell migration was detected by chamber method.The luciferase activity of cells in each group was detected by dual luciferase reporter gene.Results The expression levels of SOX4 and miR-17 mR-NA in 4 kinds of colorectal cancer cells were significantly higher than those in FHC normal colorectal mucosal epithelial cells,and the differences were statistically significant(P＜0.05).There was no significant difference in the expression level of PD-1 and PD-L1 protein and the number of cell migration between CC group and SN group(P＞0.05).The expression levels of PD-1 and PD-L1 protein and the number of cell migration in SM group were significantly higher than those in SN group,and the expression levels of PD-1 and PD-L1 protein and the number of cell migration in SI group were significantly lower than those in SM group(P＜0.05).There was no significant difference in the expression level of PD-1 and PD-L1 protein and the number of cell migration between CC group and MN group(P＞0.05).The expression levels of PD-1 and PD-L1 protein and the number of cell migration in MM group were significantly higher than those in MN group,and the expres-sion levels of PD-1 and PD-L1 protein and the number of cell migration in MI group were significantly lower than those in MM group,and the differences were statistically significant(P＜0.05).There was no significant difference in the expression level of PD-1 and PD-L1 protein and the number of cell migration between the SI group and the MI group(P＞0.05).The expression levels of PD-1 and PD-L1 protein and the number of cell migration in Ⅱ group were significantly lower than those in MI group(P＜0.05).Dual luciferase reporter gene detection,according to the results of transfection after SOX4 miR-17-3'UTR-WT luciferase activity in-creased significantly,the difference was statistically significant(P＜0.05),miR-17-3'UTR-MUT fluorescence enzyme activity has no obvious change,there was no statistically significant difference(P＞0.05).Conclusion Inhibi-tion of SOX4 expression can effectively inhibit the immune escape and migration of colorectal cancer cells,and its mechanism may be related to the inhibition of miR-17 expression.