昆明医科大学学报2024，Vol.45Issue(7)：6-13,8.DOI:10.12259/j.issn.2095-610X.S20240702
HSV-1突变株M6感染人支气管上皮细胞后对巨噬细胞介导的免疫反应的影响
Effects of HSV-1 Mutant Strain M6 on Macrophage-Mediated Immune Response after Infecting Human Bronchial Epithelial Cells
摘要
Abstract
Objective To explore the impact of herpes simplex virus type 1(HSV-1)mutant strain M6 on macrophage-mediated immune responses after infecting human bronchial epithelial cells(16HBE cells).Methods HSV-1 infection of 16HBE cells was conducted to assess cytokine alterations in the culture medium;Macrophages were co-cultured with supernatants from HSV-1-infected 16HBE cells and subsequently reintroduced into mice via tail vein infusion.Cytokine expression levels in mouse lymph nodes,changes in spleen T cell proportions,mouse neutralizing antibody levels,and specific T cell responses were measured on days 1,3,7,28,56,and 90.Results Following infection of 16HBE cells with the HSV-1 mutant strain,cytokine expression in the supernatant,associated with macrophage recruitment and activation,was elevated,albeit slightly lower than that observed in the wild-type strain group.Following the tail vein infusion experiment,the mutant strain group exhibited temporal changes in the proportions of inflammatory factors,chemokines,and T cells within the mouse lymph nodes,resulting in weaker humoral immunity compared to the wild-type strain group,yet eliciting stronger specific T cell responses,with only a few parameters showing significant differences from the wild-type strain group(P<0.05).Conclusion 16HBE cells infected with the HSV-1 mutant strain M6 can release cytokines that recruit and activate macrophages,allowing macrophages to carry specific activation information of the HSV-1 mutant strain,activating the host's immune system and inducing the host's Humoral immunity and cellular immunity.关键词
1型单纯疱疹病毒/人支气管上皮细胞/巨噬细胞/突变株Key words
Herpes simplex virus 1/Human bronchial epithelial cells/Macrophages/Mutant strain分类
医药卫生引用本文复制引用
张振潇,张晶晶,廖芸,李丹丹,李恒,刘龙丁..HSV-1突变株M6感染人支气管上皮细胞后对巨噬细胞介导的免疫反应的影响[J].昆明医科大学学报,2024,45(7):6-13,8.基金项目
国家自然科学基金资助项目(82171817) （82171817）
云南省自然科学基金资助项目(202301AS070066) （202301AS070066）
