昆明医科大学学报2024，Vol.45Issue(7)：23-29,7.DOI:10.12259/j.issn.2095-610X.S20240704
基因体甲基化在舌粘膜癌变中的变化
Changes of Genebody Methylation in Lingual Carcinogenesis
摘要
Abstract
Objective To evaluate differentially expressed genes with correlation of gene body methylation.Methods The lingual carcinogenesis was induced through the delivery of drinking water of 4-nitroquinoline-1-oxide(4NQO)with a density of 50 mg/L.The lingual mucosae specimens were harvested at 0,12,28 weeks,which represented normal,pre-cancer,and cancer individually.The microarray and methylated DNA immunoprecipitation sequencing(MeDIP-Seq)were used for the evaluation of gene expression and global DNA methylation in these specimens.The mRNA expression of Ubiquitin carboxyl-terminal hydrolase CYLD was tested with qRT-PCR in human normal and dysplasia mucosa.Results There were no statistical differences in gene body methylation among normal(C),pre-cancer(M),and cancer(E)(mean±sofMeDIP-score were 5.58±14.80,5.79±13.35,5.83±17.25 individually,H=100.75,P>0.05)There presented 145 differentially expressed genes with positive correlation to gene body methylation changes.The expression of CYLD mRNA was decreased in human dysplasia compared to normal mucosa with downregulation of gene body methylation.Conclusions There are many differentially expressed genes with a positive correlation to gene body methylations.The expression of CYLD is downregulated with the downregulation of gene body methylation in earlier stage during tongue carcinogenesis.关键词
舌癌/动物模型/甲基化/基因体/差异表达基因Key words
Tongue cancer/Animal model/Methylation/Gene body/Differentially expressed gene分类
临床医学引用本文复制引用
刘华,岳万远,邵帅,孙家平,杨莹,代晓明..基因体甲基化在舌粘膜癌变中的变化[J].昆明医科大学学报,2024,45(7):23-29,7.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[202001AY070001-253 （）
2018FE001(-216)] （-216）
