昆明医科大学学报2024，Vol.45Issue(7)：62-68,7.DOI:10.12259/j.issn.2095-610X.S20240709
宫颈癌组织中EMC6、C-myc及BCL-2的表达和预后
Expression of EMC6,C-myc and BCL-2 in Cervical Cancer Tissues and Prognosis Analysis
摘要
Abstract
Objective This study employs bioinformatics analysis to evaluate the expression,immune correlation,and diagnostic value of EMC6 gene in pan-cancer and cervical cancer,and investigate the associations of EMC6,C-myc,and BCL-2 proteins with cervical cancer and patient prognosis.Methods This study analyzed the expression of EMC6 across pan-cancer and cervical cancer datasets from TCGA and GTEx,utilizing SangerBox and Timer databases to assess its correlation with immune response and employing ROC analysis to evaluate its diagnostic potential.EMC6,C-myc,and BCL-2 protein expressions were detected in 68 cases of cervical cancer and adjacent tissues using immunohistochemistry,and their associations with clinicopathological characteristics were analyzed.The Phi coefficient method was employed to assess correlations,and the Kaplan-Meier method was used to evaluate the impact of these proteins on prognosis.Results In cervical cancer,the expression of EMC6 decreased,which significantly affected immune infiltration and had a high diagnostic accuracy(AUC=0.819).Immunohistochemistry revealed that compared to the adjacent tissues of cervical cancer,the expression of EMC6 protein decreased,while the expressions of C-myc and BCL-2 proteins were upregulated,with statistically significant differences(P<0.05).EMC6 expression showed no correlation with clinicopathological features(P>0.05),while C-myc was associated with pathological type(P<0.05),and BCL-2 was associated with age,pathological type,tumor size,and degree of differentiation(P<0.05).In cervical cancer,the expression of EMC6 was negatively correlated with C-myc and BCL-2(Phi=-0.367,P=0.002;Phi=-0.284,P=0.019),and all three were significantly associated with overall patient survival(P<0.05).Conclusions The expression of EMC6 protein decreases in cervical cancer and is negatively correlated with the expression of C-myc and BCL-2 proteins.There is a significant correlation between the expression levels of these proteins and the overall survival of patients,suggesting that they may be used as treatments for cervical cancer and Potential biomarkers for prognostic assessment.关键词
EMC6/C-myc/BCL-2/宫颈癌/细胞凋亡Key words
EMC6/C-myc/BCL-2/Cervical cancer/Apoptosis分类
医药卫生引用本文复制引用
苗春霞,王一娜,张云霞..宫颈癌组织中EMC6、C-myc及BCL-2的表达和预后[J].昆明医科大学学报,2024,45(7):62-68,7.基金项目
国家自然科学基金资助项目(81960472) （81960472）
