昆明医科大学学报2024，Vol.45Issue(7)：154-159,6.DOI:10.12259/j.issn.2095-610X.S20240723
MicroreaderTM 23HS Plex ID System中23个常染色体STR基因座在中国北方汉族人群中多态性调查
Polymorphism Investigation of 23 Autosome STR Loci in the MicroreaderTM 23HS Plex ID System in Han Population in Northern China
摘要
Abstract
Objective To obtain the distribution of allele frequency and population genetic data of 23 autosomal STR loci contained in the MicroreaderTM 23HS Plex ID System kit in the Han population of northern China,and explore its application value in forensic medicine.Methods The MicroreaderTM 23HS Plex ID System kit was used to detect the DNA of 548 unrelated samples which from the Han population in northern China.Then,the allele frequencies,heterozygosity(H)in the samples,polymorphism information content(PIC),power of discrimi-nation(DP),and probability of paternity exclusion(PE)were calculated and Hardy-Weinberg equilibrium was tested on each locus based on the genotyping data of 548 samples we collected.The cumulative individual identification ability(CDP)and cumulative non-paternity exclusion rate(CPE)of the MicroreaderTM 23HS Plex ID System were also calculated.Results A total of 260 alleles were detected in 548 unrelated samples from 23 STR loci which conformed to Hardy-Weinberg equilibrium.And the alleles frequencies ranged from 0.0009 to 0.5902,H ranged from 0.611 to 0.885,PIC ranged from 0.577 to 0.864,DP ranged from 0.815 to 0.973(mean DP was 0.922)and PE ranged from 0.089 to 0.406.At the same time,the CDP of MicroreaderTM 23HS Plex ID System was 1-3.663×10-27 and CPE was 1-2.668×10-16.Conclusion The 23 loci of MicroreaderTM 23HS Plex ID System have a good polymorphism in the north Chinese Han population,and have high application value in population genetics research,forensic personal identification,paternity testing and complex kinship identification.关键词
法医遗传学/遗传多态性/STR基因座Key words
Forensic genetics/Genetic polymorphism/STR loci分类
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石妍,江坚,陈冲,张京晶,贾莉,高知枭,李惠芬,严江伟,任贺..MicroreaderTM 23HS Plex ID System中23个常染色体STR基因座在中国北方汉族人群中多态性调查[J].昆明医科大学学报,2024,45(7):154-159,6.基金项目
上海市法医学重点实验室司法部司法鉴定重点实验室开放课题(KF202011) （KF202011）
