农业资源与环境学报2024，Vol.41Issue(4)：835-845,11.DOI:10.13254/j.jare.2023.0357
生物质炭-沼液配施条件下旱地红壤碳氮循环功能基因丰度主控因子与耦合关系
The main controlling factors and coupling relationship of functional gene abundance in carbon and nitrogen cycling in dryland red soil with biochar and biogas slurry co-application
摘要
Abstract
Carbon-nitrogen biogeochemical cycling characteristics under the combined application of biochar and biogas slurry is critical for analyzing the agricultural soil fertility enhancement.The subject of this study was typical upland red soil.Over a four-year field trial,biochar was applied at rates of 0% and 2% (C0 and C1),in combination with replacing 0% and 100% of liquid slurry(B0 and B1)to investigate changes in soil physicochemical properties,abundance of carbon and nitrogen cycling functional genes,and their coupling relationships.The combined application of biochar and biogas slurry(C1B1 treatment)increased soil pH by 1.92,reduced bulk density by 19.2%,and increased total nitrogen content by 268.6%,when compared with the control(CK)treatment.Multivariate nonlinear regression models showed that ammonium nitrogen(NH+4-N),nitrate nitrogen(NO-3-N),and water-soluble organic carbon(WSOC)were the primary factors influencing the abundance of carbon and nitrogen cycling functional genes.Furthermore,significant positive correlations were found between denitrification functional genes(nar G,nir K,nir S,and nos Z)and carbon degradation genes(chi A,pug,naglu,and gcd),carbon fixation genes(rbc L,frd A,pcc A,and mct),as well as methane metabolism genes(mxa F and pmo A).The research findings demonstrate that the combined application of biochar and biogas slurry significantly improves soil quality,and alters the abundance of functional genes in the carbon and nitrogen cycle by affecting the content of soil carbon and nitrogen forms,ultimately regulating the carbon and nitrogen cycling process of the soil.关键词
生物质炭/沼液/旱地红壤/碳氮循环/功能基因Key words
biochar/biogas slurry/upland red soil/carbon-nitrogen cycle/functional genes分类
农业科技引用本文复制引用
郑一玲,范栩妙,钟哲伦,姚光伟,林晨,顾佳艳,靳泽文,平立凤,单胜道..生物质炭-沼液配施条件下旱地红壤碳氮循环功能基因丰度主控因子与耦合关系[J].农业资源与环境学报,2024,41(4):835-845,11.基金项目
国家重点研发计划项目(2022YFE0196000) The National Key Research and Development Projects(2022YFE0196000) （2022YFE0196000）
