美洲大蠊提取物对便秘模型小鼠的影响OACSTPCD

Objective To observe the effect of different doses of Periplaneta americana extract on defecation function of slow transit constipation model mice.Methods Thirty-five male C57BL/6J mice were randomly divided into control group,model group and high,medium and low dosage groups of P.americana extract.Loperamide was given to model mice by gavage every day,and different concentrations of P.americana extract were used for intervention treatment.The fecal excretion and wet weight of mice in each group were recorded after administration.Before the end of the experiment,the feces of mice in each group were collected and the changes of intestinal flora were analyzed by 16S rRNA.At the same time,the first defecation time and small intestine propulsion rate of mice in each group were measured.Results Compared with the control group,the wet weight of feces,the number of particles and the intestinal propulsion rate of the model group were significantly reduced.However,the time of red stool excretion was significantly prolonged(P<0.01).Compared with the model group,the wet weight of feces,the number of particles and the small intestine propulsion rate of mice in each P.americana treatment group increased significantly,while the time of red stool excretion decreased significantly(P<0.05 or P<0.01).16S rRNA analysis showed that P.americana extract could significantly increase the abundance of Lachnospiraceae NK4A136 and Bacteroides acidifaciens,Alistipes and Rikenella microfusus DSM 15922 in intestinal feces of constipation mice.Conclusion P.americana extract can alleviate the constipation induced by loperamide in mice,and its mechanism is related to the regulation of intestinal flora.