基于HS-GC-Orbitrap联用定性与定量测定活血止痛膏挥发性成分OACSTPCD
Qualitative and quantitative detection of volatile constituent in Huoxue Zhitong Ointment based on HS-GC-Orbitrap
目的 建立活血止痛膏中挥发性成分定性、定量检测方法.方法 采用顶空进样-气相色谱-静电场轨道阱质谱(HS-GC-Orbitrap)技术进行分析.样品除去背膜,以乙腈为溶剂,顶空进样;以高纯氦气为载气,TG-5SILMS色谱柱,程序升温,恒压模式,电子轰击源(EI源),全扫描监测模式测定.结果 可定性活血止痛膏中 5 味药的 12 个主要成分,同时定量测定樟脑、异龙脑、龙脑、薄荷脑、水杨酸甲酯和丁香酚等 6 个成分.结论 研究建立的方法提升了活血止痛膏质量控制水平,操作简便.
Objective To establish a qualitative and quantitative method for the determination of volatile components in Huoxue Zhitong Ointment.Methods Headspace sampling-gas chromatography-electrostatic field orbital well mass spectrometry was used for analysis.The back film was removed,acetonitrile was used as solvent,and the sample was injected into headspace,TG-5SILMS column with high purity helium as carrier gas,programmed temperature,constant pressure mode,electron bombardment source(EI),and total scanning monitoring mode.Results Twelve main components of Huoxue Zhitong Ointment were identified,and six main active components including camphor,isoborneol,borneol,menthol,methyl salicylate and eugenol were quantitatively determined.Conclusion The methods can improve the quality control level of Huoxue Zhitong Ointment,which are easy to operate.
刘鹏鸣;李锋武;杨会鸽
西安市食品药品检验所,陕西 西安 710119
药学
活血止痛膏樟脑异龙脑龙脑薄荷脑水杨酸甲酯丁香酚静电场轨道阱质谱
Huoxue Zhitong OintmentCamphorIsoborneolBorneolMentholMethyl salicylateEugenolElectrostatic field orbital well mass spectrometry
《药学研究》 2024 (006)
559-563,593 / 6
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