实验动物与比较医学2024，Vol.44Issue(3)：321-327,7.DOI:10.12300/j.issn.1674-5817.2023.168
啮齿类实验动物健康监测用脏垫料哨兵动物法和排风粉尘PCR法比较
Comparison of Methods between Soiled Bedding Sentinels and Exhaust Air Dust PCR for Health Monitoring of Rodent Laboratory Animals
摘要
Abstract
The microbiological quality of laboratory animals is crucial for the validity and reproducibility of scientific research data,as well as human health and animal welfare.Currently,individual ventilation cages(IVC)have become the mainstream feeding system for rodent laboratory animals.The most commonly used pathogen monitoring method for this feeding system is soiled bedding sentinels(SBS).This method monitors the microbial carrying status of mouse colony through indirect contact and delayed feedback.It can effectively monitor pathogens transmitted via the fecal-oral route,such as mouse hepatitis virus and reovirus.However,this method has difficulty detecting pathogens mainly transmitted through aerosols or direct contact,such as Sendai virus and Pasteurella pneumotropica.The exhaust air dust(EAD)-PCR monitoring method involves swab sampling in the IVC exhaust ducts to monitor the corresponding racks of the ducts;swab sampling before the prefiltration of the host to monitor the entire IVC rack;and EAD collection device sampling to monitor all racks connected to the same host.Different IVC manufacturers have developed corresponding EAD collection devices for their respective IVC systems,making operations convenient and standardization easy.Compared with the SBS method,the EAD-PCR method significantly improves detection rate and timeliness,with the fastest detection possible after one week of exposure.It can serve as a supplement or replacement for the SBS method.Currently,increasing evidence supports that EAD-PCR testing is a more reliable,sensitive,and cost-effective monitoring method,and is more beneficial to animal welfare.This article reviews the application progress of these two methods for monitoring pathogens,analyzes the existing limitations of the EAD-PCR method,and proposes solutions based on its implementation in our laboratory and examination units.The EAD-PCR method helps reduce the number of live sentinel animals used in pathogen monitoring,in order to better maintain the"3Rs"principle of laboratory animal welfare.关键词
啮齿类实验动物/健康监测/脏垫料哨兵/排风粉尘PCR监测Key words
Rodent laboratory animals/Health monitoring/Soiled bedding sentinels/Exhaust air dust-PCR monitoring分类
生物科学引用本文复制引用
于灵芝,魏晓锋,黎明,孔志豪..啮齿类实验动物健康监测用脏垫料哨兵动物法和排风粉尘PCR法比较[J].实验动物与比较医学,2024,44(3):321-327,7.基金项目
上海实验动物研究中心科技创新计划新星项目"木糖葡萄球菌快速分子检测方法的建立及应用"(2024NS05) （2024NS05）
上海市科技计划项目资助"上海市水生实验动物专业技术服务平台"(22DZ2291200) （22DZ2291200）
