姜黄素联合花色苷对睡眠剥夺合并动脉粥样硬化大鼠模型的影响OACSTPCD

In order to study the protective effects and mechanism of curcumin(Cur)combined with antho-cyanin(Ant)on the rat model of sleep deprivation(SD)and atherosclerosis(AS),42 rats were randomly di-vided into seven groups,including normal control group,AS model group,AS+SD model group,AS+plat-form control group,Cur protection group,Ant protection group,and Cur+Ant co-protection group.All the rats except those in normal control group were fed with high-fat chow for 12 weeks to construct an AS model.Then the rats in the AS+SD model group and three protection groups were treated with SD for 2 weeks using the modified multi-platform method(MMPM).At the end of modeling,the rats in the protection groups were treated with the corresponding drug administration.The serum indexes were detected,the changes in the heart and thoracic aorta were observed by hematoxylin-eosin(HE)staining,atherosclerotic plaque deposition in the arch of the thoracic aorta was observed by Oil Red O staining,and the expression levels of Bcl-2,caspase-3 and caspase-9 proteins in the myocardial tissues were detected by Western-blot.The results showed that,compared with the normal control group,both the AS model and AS+SD model groups had ele-vated levels of serum interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),total cholesterol(TC),triglyce-ride(TG),and fasting blood glucose(FBG)(P＜0.05),with a large number of disordered cardiomyocytes and aortic endothelial cells,and a large number of infiltrated inflammatory cells,showing significant atheroscle-rotic injury.Furthermore,the AS+SD model group had a more severely damage degree than the AS model group.Compared with those in the model groups,rats in the protection groups had an alleviated damage de-gree after treated by either Cur or blueberry Ant,and had no significant damage and lesion in the heart and aorta when the two drugs were co-administered.In addition,the expression levels of caspase-3 and cas-pase-9 proteins in myocardial tissues in the model groups were significantly higher than those in the normal control group(P＜0.05),and the levels of Bcl-2 proteins were significantly lower than that in the normal con-trol group(P＜0.05).Following the drug intervention,caspase-3 and caspase-9 levels were significantly down-regulated(P＜0.05),and Bcl-2 levels were significantly up-regulated(P＜0.05)in the protection groups.The results indicated that SD would aggravate the development of AS in rats,however,both Cur and Ant could repair the damage of cardiomyocytes in rats with SD and AS,and the combination of the two drugs had a better repair effect.