生命科学研究2024，Vol.28Issue(3)：206-214,9.DOI:10.16605/j.cnki.1007-7847.2023.11.0204
七氟醚通过STING/AMPK信号通路减轻小鼠肝脏缺血再灌注损伤的研究
Sevoflurane Attenuates Hepatic Ischemia-reperfusion Injury in Mice via STING/AMPK Signaling Pathway
摘要
Abstract
To explore the effects and mechanisms of sevoflurane anesthesia on hepatic ischemia-reperfusion injury(HIRI)in mice,healthy adult male C57 mice were randomly divided into three groups,including sham group(Sham),HIRI+pentobarbital sodium group(HIRI+Pent),and HIRI+sevoflurane group(HIRI+Sevo),10 in each group.The mouse model of partial(70％)HIRI was constructed by laparotomy.After liver ischemia for 60 min and reperfusion for 3 h,mice were sacrificed for sample collection.The pathological damage of liver tissue was observed with hematoxylin-eosin(HE)staining,the content of myeloperoxidase(MPO)was detected by immunofluorescence staining,and apoptosis in tissue was detected using the terminal deoxynu-cleotidyl transferase(TdT)-mediated dUTP-biotin nick end labeling(TUNEL)method.The expression of in-flammation-related factors was detected by both enzyme-linked immunosorbent assay(ELISA)and polymerase chain reaction(PCR).The expression of stimulator of interferon genes(STING),AMP-activated protein kinase(AMPK)and other related proteins was detected by Western-blot.Serum alanine transaminase(ALT),aspar-tate transaminase(AST)and lactate dehydrogenase(LDH)were detected by biochemical analyzer.Meanwhile,the contents of reduced glutathione(GSH),lipid peroxide malondialdehyde(MDA)and superoxide dismutase(SOD)were measured by thiobarbituric acid(TBA)colorimetry,xanthine oxidase method,and dithio-dinitro-benzoic acid method,respectively.The results showed that,compared with mice in Sham group,after ische-mia-reperfusion,mice in HIRI+Pent group had significantly increased serum ALT and AST levels,large in-farcted area in liver tissue,significantly enhanced apoptosis elevated STING and nuclear factor-KB(NF-κB)expression levels,significantly decreased AMPK expression,significantly increased levels of inflammatory factors tumor necrosis factor-a(TNF-a),interleukin(IL)-1β,IL-6,IL-18,and MPO,and significantly dif-ferent levels of the oxidative stress markers GSH,MDA and SOD.However,after ischemia-reperfusion,mice in HIRI+Sevo group exhibited alleviated degrees of all the above changes,with statistical significance(P＜0.05).The study demonstrated that sevoflurane can partially reduce HIRI in mice through STTNG/AMPK signaling pathway.关键词
七氟醚/肝脏缺血再灌注损伤(HIRI)/干扰素基因刺激因子(STING)/AMP活化蛋白激酶(AMPK)Key words
sevoflurane/hepatic ischemia-reperfusion injury(HIRI)/stimulator of interferon genes(STING)/AMP-activated protein kinase(AMPK)分类
生物科学引用本文复制引用
张海霞,程志军,李辉,季惠..七氟醚通过STING/AMPK信号通路减轻小鼠肝脏缺血再灌注损伤的研究[J].生命科学研究,2024,28(3):206-214,9.基金项目
上海市崇明区重点学科(扶持学科)计划项目 （扶持学科）
