广粪蚊共生菌发酵液对宿主嗅觉行为反应的影响及其挥发物成分分析OACSTPCD

Coboldia fuscipes(Meigen)is one of the main pests of edible fungi.The symbiotic bacteria in the insect may have a potential biocontrol value and be used in green control of the insect.Herein,the Y-type olfactory tester was used to test the olfactory behavior responses of the C.fuscipes adults to the fermentation broths of 21 symbiotic bacteria,which belong to 16 genera in 14 families,in 3 test periods of 10 min,20 min and 30 min.The results showed that the fermentation broths of Alcaligenes aquatilis,Citrobacter sp.and Paenochrobactrum glaciei had a significant attractant effect on the adults,while the fermentation broth of Bacillus safensis had a significant repellent effect on the adults.The relative attractive rates of these four broths reached 20.00％,13.75％,15.00％and-18.75％in 30 min,respectively.Then the volatiles of the four fermentation broths were analyzed by gas chromatography-mass spectrometry(GC-MS).The results showed that 28 compounds with the relative content higher than 2％were identified.In both A.aquatilis and P.glaciei broths,benzaldehyde is the most abundant volatile,followed by pyrazine,2,5-dimethyl-and 1,10-di-cyanodecane,respectively.The relative content of formamide,N-phenyl-was the highest in C.sp.,followed by butanoic acid,3-methyl-,3-methylbutyl ester.The relative content of butanoic acid,3-methyl-,ethyl ester was the highest in B.safensis,followed by butanoic acid,3-methyl-,1-methylethyl ester.In conclu-sion,the symbiotic bacteria with attractant or repellent effect on adult C.fuscipes were identified,and spe-cific components in the volatiles were determined,which would benefit the develop-ment of attractants or re-pellents for C.fuscipes.