生命科学研究2024，Vol.28Issue(3)：232-240,9.DOI:10.16605/j.cnki.1007-7847.2023.11.0215
广粪蚊共生菌发酵液对宿主嗅觉行为反应的影响及其挥发物成分分析
Effects of Fermentation Broths of Symbiotic Bacteria in Coboldia fuscipes(Meigen)on Host Olfactory Behavioral Responses and Analysis of Volatiles
摘要
Abstract
Coboldia fuscipes(Meigen)is one of the main pests of edible fungi.The symbiotic bacteria in the insect may have a potential biocontrol value and be used in green control of the insect.Herein,the Y-type olfactory tester was used to test the olfactory behavior responses of the C.fuscipes adults to the fermentation broths of 21 symbiotic bacteria,which belong to 16 genera in 14 families,in 3 test periods of 10 min,20 min and 30 min.The results showed that the fermentation broths of Alcaligenes aquatilis,Citrobacter sp.and Paenochrobactrum glaciei had a significant attractant effect on the adults,while the fermentation broth of Bacillus safensis had a significant repellent effect on the adults.The relative attractive rates of these four broths reached 20.00％,13.75％,15.00％and-18.75％in 30 min,respectively.Then the volatiles of the four fermentation broths were analyzed by gas chromatography-mass spectrometry(GC-MS).The results showed that 28 compounds with the relative content higher than 2％were identified.In both A.aquatilis and P.glaciei broths,benzaldehyde is the most abundant volatile,followed by pyrazine,2,5-dimethyl-and 1,10-di-cyanodecane,respectively.The relative content of formamide,N-phenyl-was the highest in C.sp.,followed by butanoic acid,3-methyl-,3-methylbutyl ester.The relative content of butanoic acid,3-methyl-,ethyl ester was the highest in B.safensis,followed by butanoic acid,3-methyl-,1-methylethyl ester.In conclu-sion,the symbiotic bacteria with attractant or repellent effect on adult C.fuscipes were identified,and spe-cific components in the volatiles were determined,which would benefit the develop-ment of attractants or re-pellents for C.fuscipes.关键词
广粪蚊/共生菌/Y型嗅觉测试仪/嗅觉行为/气相色谱-质谱联用仪(GC-MS)/挥发物成分分析Key words
Coboldia fuscipes(Meigen)/symbiotic bacteria/Y-type olfactory tester/olfactory behavior/gas chromatography-mass spectrometry(GC-MS)/analysis of volatiles分类
生物科学引用本文复制引用
李炳宏,王剑峰,王丹丹..广粪蚊共生菌发酵液对宿主嗅觉行为反应的影响及其挥发物成分分析[J].生命科学研究,2024,28(3):232-240,9.基金项目
国家自然科学基金资助项目(31672332) （31672332）
辽宁省自然科学基金项目(2019-ZD-0539) （2019-ZD-0539）
