生命科学研究2024，Vol.28Issue(3)：247-257,11.DOI:10.16605/j.cnki.1007-7847.2023.10.0196
基于血餐iDNA技术对武陵洞蛭专吸蝠血性的研究
Study of Sinospelaeobdella wulingensis Exclusively-Batblood-Sucking Based on Bloodmeal iDNA Technology
摘要
Abstract
Wuling cave leeches(Sinospelaeobdella wulingensis),the type species of Sinospelaeobdella as well as a kind of troglobites,live with cave-dwelling bats in some karstic caves of relatively stable environmental conditions.They feeds on the blood of cave-dwelling bats,and live in the roof of the caves at all their lives.To explore the exclusively-batblood-sucking of the leeches,from August 2022 to August 2023,appropriate samples and relevant data of micro-habitat characteristics were collected by conventional ways in six caves of S.wulingensis in Xiangxi Tujiazu and Miaozu Autonomous Prefecture,Hunan Province,and a total of 106 host bloodmeal samples of S.wulingensis were identified by iDNA technology.The results showed that:1)The cave leeches usually inhabited some wet and rough cave ceilings,which have a 0.2～0.4 cm thick layer of rock/mud debris,and the population distribution of the leeches was closely related to the roost sites of cave-dwelling bats;2)The hosts of the cave leeches included 11 species from 5 genera and 5 families of Chiroptera,among which Rhinolophus sinicus,Hipposideros armiger and Myotis altarium were the main blood supplier;3)The food diversity index and niche breadth of each S.wulingensis population were lower than the overall levels,while these characteristic indicators in winter were higher than those in other sea-sons.In conclusion,Wuling cave leeches are mainly distributed in the cave roof near where bats often roost.Due to long-term adaptation to cave life,they have evolved a specialized feeding habit and ecological beha-vior,that is,exclusively-batblood-sucking.Further studying the bat blood-sucking nature of the leeches will help to explore the evolutionary path of leech food diversity and reveal the behavioral ecological mechanism of haematophagous leech feeding habits.关键词
武陵洞蛭/血餐/iDNA技术/洞栖性蝙蝠/专吸蝠血性Key words
Sinospelaeobdella wulingensis/bloodmeal/iDNA technology/cave-dwelling bat/exclusively-batblood-sucking分类
生物科学引用本文复制引用
吴清姐,向冬琴,向海洋,廖金屏,李金美,刘志霄..基于血餐iDNA技术对武陵洞蛭专吸蝠血性的研究[J].生命科学研究,2024,28(3):247-257,11.基金项目
国家自然科学基金资助项目(32160241) （32160241）
国家自然科学基金重点国际(地区)合作与交流项目(31961123003)委托子项目 （地区）
湖南省自然科学基金项目(2021JJ30554) （2021JJ30554）
吉首大学研究生科技创新项目(Jdy22062) （Jdy22062）
