实用医学杂志2024，Vol.40Issue(14)：1928-1934,7.DOI:10.3969/j.issn.1006-5725.2024.14.005
白术内酯Ⅰ在胃癌细胞中抑制输出蛋白T的作用和机制
Role and mechanism of XPOT inhibition by atractylenolide I in gastric cancer cells
摘要
Abstract
Objective This study aimed to investigate the role and mechanism of atractylenolide Ⅰ in inhibiting XPOT proliferation and invasion in gastric cancer cells.Methods This study included exploration of XPOT expression in gastric cancer tissues,analysis of gene expression data from GC patients in TCGA and GEO databases,as well as various cellular assays to detect the ability of cancer cells to proliferate,migrate,and invade.Protein expression levels of XPOT,SKP2,CyclinA,and P27 mRNA were also detected by qPCR and Western Blot.Results Analysis confirmed that XPOT was highly expressed in gastric cancer tissues,indicating a poor prognosis.In vitro studies revealed that AT-1 inhibits the proliferation and invasion ability of GC cells;XPOT down-regulation also inhibits these abilities.Furthermore,AT-1 down-regulates the expression of XPOT which then regulates SKP2,P27,and CyclinA-ultimately inhibiting the proliferation and invasion of gastric cancer cells through the regulation of the XPOT pathway.Conclusion The overexpression of XPOT in gastric cancer tissues can indicate a poor prognosis.Atractylenolide Ⅰ down-regulates the XPOT-regulated ubiquitination-proteasome pathway to inhibit proliferation and invasion of gastric cancer cells.关键词
白术内酯Ⅰ/胃癌/输出蛋白T/泛素化-蛋白酶体途径Key words
atractylodes macrocephala lactone Ⅰ/gastric cancer/XPOT/ubiquitination proteasome pathway分类
医药卫生引用本文复制引用
张艺,马芳琪,魏思媛,李学军..白术内酯Ⅰ在胃癌细胞中抑制输出蛋白T的作用和机制[J].实用医学杂志,2024,40(14):1928-1934,7.基金项目
安徽省科技惠民专项(编号:2022e07020023) （编号:2022e07020023）
安徽省自然科学基金项目(编号:2008085MH265) （编号:2008085MH265）
安徽省名中医及基层名中医工作室建设项目(编号:中发展[2020]10号) （编号:中发展[2020]10号）
