深圳大学学报（理工版）2024，Vol.41Issue(4)：453-462,10.DOI:10.3724/SP.J.1249.2024.04453
羟基磷灰石-β-环糊精复合微球的合成与性能
Synthesis and performance of hydroxyapatite-β-cyclodextrin composite microspheres
摘要
Abstract
Hydroxyapatite(HA)is a widely used drug loading material,and combining HA with related materials to form drug loaded microspheres can effectively alleviate the phenomenon of drug burst release.For this purpose,the synthesis was involved using sodium dodecyl sulfonate(SDS)and proline(Pro)as templates through a hydrothermal precipitation method to create calcium carbonate microspheres.Subsequently,these microspheres were combined with β-cyclodextrin via ion exchange in a hydrothermal process to create a cavity structure.The effects of varying synthesis conditions on the microsphere morphology were investigated.The results showed that when the content of cyclodextrin was 20%-30% and the hydrothermal reaction time was 6 h,HA-β-CD composite microspheres had well-defined morphology and good dispersion.Characterization using X-ray diffraction,scanning electron microscope,UV-Vis spectrophotometer confirmed the uniform particle size distribution(6.4-13.9 µm)and presence of cavity structures in the HA-β-CD microspheres.Drug loading studies using curcumin as a model drug demonstrated that HA-β-CD microspheres achieved enhanced the drug loading capacity(5.03%±0.37% )and encapsulation efficiency(37.67%±1.37% )when β-cyclodextrin content was optimized at 30% .Vitro drug release tests indicated sustained release behavior,effectively mitigating burst release observed in traditional HA microspheres,thereby prolonging the drug efficacy.At the same time,the formation mechanism and cytotoxicity of drug loaded microspheres were analyzed and explored.The results showed that HA-β-CD drug loaded composite microspheres were formed through the encapsulation of HA,β-CD,and drugs,which can achieve delayed drug release and significantly improve the sustained release performance of drug loaded microspheres.Moreover,the test results showed that HA-β-CD composite microspheres have almost no toxicity to cell proliferation.HA-β-CD composite microspheres address limitations of drug-loaded pure HA microspheres such as rapid drug release,low drug loading,short duration of action,highlighting their significant medical value and application potential.关键词
生物医用材料/复合微球/羟基磷灰石/β-环糊精/模板法/载药性能/姜黄素Key words
biomedical materials/composite microspheres/hydroxyapatite(HA)/β-cyclodextrin(β-CD)/template method/drug loading performance/curcumin分类
医药卫生引用本文复制引用
袁秋华,钟骏熙,夏贤友,王涛,余宏,代小毅,吴晓婉,吴文珊,简友亮,杨袁,李瑞龙..羟基磷灰石-β-环糊精复合微球的合成与性能[J].深圳大学学报（理工版）,2024,41(4):453-462,10.基金项目
Shenzhen Natural Science Fund(The Stable Support Plan Program)(20231121202105001) （The Stable Support Plan Program）
National Nataral Science Fundation of China(21471102) （21471102）
Shenzhen Basic Research Foundation(JCYJ20190808110613626) 深圳市高校稳定支持计划面上资助项目(2023112120210 5001) （JCYJ20190808110613626）
国家自然科学基金资助项目(21471102) （21471102）
深圳市基础研究计划资助项目(JCYJ20190808110613626) （JCYJ20190808110613626）
