天津中医药2024，Vol.41Issue(7)：922-928,7.DOI:10.11656/j.issn.1672-1519.2024.07.20
丹参多酚酸盐调控Nrf2/HO-1信号通路对膜性肾病大鼠氧化应激的影响
Effect of Nrf 2/HO-1 signaling regulated by salvia miltiorrhiza polyphenols on oxidative stress in rats with membranous nephropathy
摘要
Abstract
[Objective]To investigate the effect of miltiorrhiza polyphenols on oxidative stress in membranous nephropathy(MN)rats based on the serum nuclear transcription factor 2/hemoglobin oxygenase-1(Nrf 2/HO-1)signaling pathway.[Methods]The 80 SD male rats were randomly selected as normal control group,and the remaining 60 rats were treated with tail vein injection of cated bovine serum albumin(C-BSA)to construct the MN model.MN rats were randomly divided into model group,benenapril hydrochloride(10 mg/kg),and polyphenols were divided into low,medium and high dose groups(16.7,33.3 and 66.7 mg/kg).Drug gavage for 4 weeks,1 time/d,and equal volume of saline gavage in normal and model groups.Ururine protein quantification at 24 h(24 h UTP).After the administration of the drug,blood urea nitrogen(BUN),serum creatinine(Scr),triglyceride(TG),total cholesterol(TC),total protein(TP),albumin(ALB)levels were measured;the renal histopathology was observed by silver overdate-hexidamine(PASM);the deposition of immunoglobulin G(IgG)and complement C3 was detected by immunofluorescence;the expression of superoxide dismutase(SOD)and malondialdehyde(MDA)was detected by enzyme-linked immunosorbent assay(ELISA);the expression of Nrf 2 and HO-1 was observed by protein immunoblotting(Western Blot).[Results]Compared with the normal group,the glomeruli in the model group showed increased size,basement membrane thickening,the formation of"nail process",diffuse deposition of complement C3 and IgG along the capillary haptics,UTP,serum TG and TC levels at 24 h significantly increased(P＜0.01),and TP and ALB levels significantly decreased(P＜0.01).There was no significant difference between BUN and SCr;SOD expression was significantly decreased(P＜0.0 1);MDA expression was significantly increased(P＜0.01);Nrf 2,HO-1 mRNA and protein expression were significantly decreased(P＜0.01).Compared with the model group,24 h UTP,serum TG,TC were significantly decreased(P＜0.05 or P＜0.01),TP,ALB(P＜0.05);renal pathological damage was significantly improved;SOD(P＜0.01),MDA(P＜0.01);Nrf 2,HO-1 mRNA and protein(P＜0.05 or P＜0.01).[Conclusion]Salvia miltiorrhiza polyphenols may regulate the Nrf 2/HO-1 signaling pathway,and thus protect the kidney and delay the disease progression.关键词
丹参多酚酸盐/膜性肾病/氧化应激/Nrf2/HO-1信号通路Key words
miltiorrhiza polyphenols/membranous nephropathy/oxidative stress/Nrf 2/HO-1 signaling pathway分类
医药卫生引用本文复制引用
潘华荣,高圣柠,申晨卉,檀淼,唐淼,王兆黎,檀金川..丹参多酚酸盐调控Nrf2/HO-1信号通路对膜性肾病大鼠氧化应激的影响[J].天津中医药,2024,41(7):922-928,7.基金项目
河北省自然科学基金项目(2019423037) （2019423037）
河北省研究生创新资助项目(XCXZZSS2023041). （XCXZZSS2023041）
