天津中医药2024，Vol.41Issue(7)：929-934,6.DOI:10.11656/j.issn.1672-1519.2024.07.21
黄精多糖对妊娠期高血压模型大鼠胎盘组织TGF-β/Smad信号通路的作用研究
Study on the effect of polygonatum polysaccharides on the TGF-β/Smad signal pathway in placental tissue of pregnancy induced hypertension rats
摘要
Abstract
[Objective]To investigate the impacts and mechanism of polygonatum polysaccharide on placental function in pregnancy induced hypertension model rats.[Methods]Pregnant female rats were randomly grouped into control group,model group,polygonatum polysaccharide(low,medium and high)dose groups(100,200,400 mg/kg)and magnesium sulfate group(100 mg/kg),with 10 rats in each group.In addition to the control group,rats in other groups were subcutaneously injected with L-arginine methyl ester to build the hypertensive model of pregnancy.Blood pressure,24-hour proteinuria,fetal rat body mass and placenta weight were measured;hematoxylin-eosin(HE)was applied to detect the histopathology of placenta;the contents of interleukin(IL)-6,IL-10 and tumor necrosis factor-α(TNF-α)in placenta were detected by enzyme linked immunosorbent assay(ELISA);Western Blot was applied to detect the expression of tight junction protein-1(ZO-1),blocking protein(OCLN),vascular endothelial growth factor(VEGF),transforming growth factor-β1(TGF-β1),p-Smad3 and Smad3 proteins in placenta.[Results]Compared with the control group,the blood pressure,24-hour urine protein,IL-6,TNF-α contents and TGF-β1,p-Smad3 protein expression in the model group were significantly higher;the body weight,placenta weight,IL-10 content and the expression of ZO-1,OCLN and VEGF protein of fetal rats were significantly lower(P＜0.05),and pathological damage occurred in placenta tissue.Compared with the model group,the blood pressure,24-hour urine protein,IL-6,TNF-α contents and TGF-[31,p-Smad3 protein expression of rats in administration groups were significantly lower,the body weight,placenta weight,IL-10 content and the expression of ZO-1,OCLN and VEGF protein of fetal rats were significantly higher(P＜0.05),and the pathological damage of placenta was alleviated.[Conclusion]Polygonatum polysaccharide can inhibit the activation of TGF-β/Smad signal pathway in the placenta of pregnancy induced hypertension model rats and improve the placental dysfunction.关键词
妊娠期高血压/胎盘功能/黄精多糖/TGF-β/Smad信号通路Key words
pregnancy induced hypertension/placental function/polygonatum polysaccharide/TGF-β/Smad signal pathway分类
医药卫生引用本文复制引用
周芳芳,刘宇,马双玲,李晓倩,张新宁,李辞妹,毕晶晶,李国芸..黄精多糖对妊娠期高血压模型大鼠胎盘组织TGF-β/Smad信号通路的作用研究[J].天津中医药,2024,41(7):929-934,6.基金项目
2019年度河南省医学科技攻关计划(联合共建)项目(LHGJ20191328). （联合共建）
