基于网络药理学及分子对接技术探讨过敏煎对特应性皮炎的作用机制OA

Objective To explore the mechanism of Guominjian on atopic dermatitis(AD)based on net-work pharmacology and molecular docking techniques.Methods The active ingredients and corresponding targets of Guominjian were retrieved from TCMSP database,and AD disease related targets were obtained from OMIM,GeneCards and DrugBank database.Cytoscape3.7.2 software was used to construct the Chinese medicine component target network and STRING platform for protein interaction network analysis.GO func-tion and KEGG signal pathway database were used for enrichment analysis,active ingredient-target-pathway network was constructed,and molecular docking was performed with AutoDockVina software.Results There were 123 common targets of Guominjian and AD.GO functional enrichment analysis involved 641 items of bi-ological processes,68 items of cell components and 129 items of molecular functions.The signaling pathways including MAPK,NF-κB,TNF-α,JAK-STAT and PI3K-Akt,etc.were selected by enrichment analysis of KEGG signaling pathways.The results of molecular docking showed that quercetin and kaempferol had high affinity with FOS,TNF,TGFB1,HIF-1A and IL 1 A.Conclusion Guominjian can treat AD from multi-target and multi-pathway,which providing theoretical basis for further basic experimental research.