妊娠晚期感染B族链球菌孕妇早产的危险因素及其pbp2x基因突变分析OA

Objective To analyze the risk factors for preterm birth in pregnant women with group B streptococcus(GBS)infection in a hospital and investigate the pbp2x gene mutation of GBS in pregnant women with preterm birth.Methods A total of 199 pregnant women with GBS infection who delivered in the hospital from January 2022 to May 2023 were selected and divided into the observation group and the control group according to whether they were preterm or not.Binary logistic regression model and receiver operating characteristic curve(ROC)were used to analyze the risk factors of preterm birth in pregnant women with GBS infection.First generation sequencing of pbp2x gene was performed on GBS samples from pregnant women with preterm birth.Results Among the 199 cases pregnant women with GBS infection,41(20.6％)had pre-term birth.Logistic regression analysis showed that age[odds ratio(OR)=1.151,P=0.004]and the number of abnormal items in oral glucose tolerance test(OGTT)(OR=2.995,P＜0.001)were independent risk fac-tors for preterm birth in pregnant women with GBS infection.The area under the curve(AUC)of the combi-nation of the two risk factors for preterm birth was 0.783,the best sensitivity was 75.6％,and the specificity was 69.0％,which was higher than that of single risk factor.Three missense mutations(1129G＞A,1148T＞G,1528A＞G)in pbp2x gene were found by Sanger sequencing.No mutation was found that could reduce the susceptibility of GBS to penicillin.Conclusion Age and the number of abnormal items in OGTT are risk fac-tors for preterm birth in pregnant women with GBS infection,and the combination of the two factors is better than each one alone in predicting preterm birth.No mutation of pbp2x gene affecting penicillin sensitivity has been found in GBS from pregnant women with preterm birth.