中国当代儿科杂志2024，Vol.26Issue(7)：683-689,7.DOI:10.7499/j.issn.1008-8830.2311151
运用尿蛋白组学技术探索凝血酶与炎症因子相互作用参与IgA血管炎发病的机制
Exploring the mechanism of IgA vasculitis pathogenesis through the interaction of thrombin and inflammatory factors using urinary proteomics
摘要
Abstract
Objective To explore the evidence,urinary biomarkers,and partial mechanisms of hypercoagulability in the pathogenesis of IgA vasculitis(IgAV).Methods Differential expression of proteins in the urine of 10 healthy children and 10 children with IgAV was screened using high-performance liquid chromatography-tandem mass spectrometry,followed by Reactome pathway analysis.Protein-protein interaction(PPI)network analysis was conducted using STRING and Cytoscape software.In the validation cohort,15 healthy children and 25 children with IgAV were included,and the expression levels of differential urinary proteins were verified using enzyme-linked immunosorbent assay.Results A total of 772 differential proteins were identified between the IgAV group and the control group,with 768 upregulated and 4 downregulated.Reactome pathway enrichment results showed that neutrophil degranulation,platelet activation,and hemostasis pathways were involved in the pathogenesis of IgAV.Among the differential proteins,macrophage migration inhibitory factor(MIF)played a significant role in neutrophil degranulation and hemostasis,while thrombin was a key protein in platelet activation and hemostasis pathways.PPI analysis indicated that thrombin directly interacted with several proteins involved in inflammatory responses,and these interactions involved MIF.Validation results showed that compared to healthy children,children with IgAV had significantly higher urine thrombin/creatinine and urine MIF/creatinine levels(P<0.05).Conclusions Thrombin contributes to the pathogenesis of IgAV through interactions with inflammatory factors.Urinary thrombin and MIF can serve as biomarkers reflecting the hypercoagulable and inflammatory states in children with IgAV.关键词
IgA血管炎/蛋白质组学/凝血酶/高凝/炎症/儿童Key words
IgA vasculitis/Proteomics/Thrombin/Hypercoagulability/Inflammation/Child引用本文复制引用
刘萌萌,侯改灵,杨晓青,张秋爽,梅晓峰,丁樱,宋兰,黄岩杰..运用尿蛋白组学技术探索凝血酶与炎症因子相互作用参与IgA血管炎发病的机制[J].中国当代儿科杂志,2024,26(7):683-689,7.基金项目
国家自然科学基金面上项目(82174187) （82174187）
中原科技创新领军人才项目(234200510028) （234200510028）
河南省中医药学科领军人才项目([2021]No.8) （[2021]No.8）
河南省卫生健康委国家中医临床研究基地科研专项(2022JDZX014) （2022JDZX014）
河南省特色骨干学科中医学学科建设项目(STG-ZYXKY-2020008). （STG-ZYXKY-2020008）
