中国临床医学2024，Vol.31Issue(3)：428-432,5.DOI:10.12025/j.issn.1008-6358.2024.20240340
基于广义估计方程评估尿液混匀程度对慢性肾脏病患者24 h尿蛋白定量的影响
Effect of urine mixing degree on 24-hour urinary total protein in patients with chronic kidney disease based on generalized estimating equation
摘要
Abstract
Objective To explore the effect of urine mixing degree on 24-hour urinary total protein(24 h UTP)in patients with chronic kidney disease(CKD).Methods From October 1,2023 to December 31,2023,30 hospitalized patients who needed to complete 24 h UTP testing in Zhongshan Hospital,Fudan University were selected.A 5 L unified container was used to collect urine for 24 hours.After collection and one hour's standing,the urine sample was divided into upper,middle,and lower equal parts according to volume,which was defined as direct-sampling group.Then,the urine samples were fully mixed with a magnetic stirrer and sampled again according to the above-mentioned three-equal sampling method,which was defined as mixed-sampling group.The generalized estimating equation was used to compare the urinary protein concentration before and after mixing and at different sampling location.Results The results of generalized estimating equation showed that after controlling the variable"sampling position",there was no significant difference in urinary protein concentration between the direct-sampling group and the mixed-sampling group.After controlling the variable"mixing method",there was still no significant difference in urinary protein concentration at different sampling positions.After adjusting the covariates such as age,gender,and estimated glomerular filtration rate(eGFR),the results were consistent.Conclusions With standard protocol,the entire 24-hour urine sample is a relatively even-distributed solution.After the total urine collection is completed,the temporary sample can be directly extracted from any level of the original urine within 1 hour,and the urine protein concentration of the sample multiplied by the urine volume can reflect the 24 h UTR.关键词
慢性肾脏病/24h尿蛋白定量/广义估计方程/混匀方式/采样位置Key words
chronic kidney disease/24-hour urinary total protein/generalized estimating equation/mixing method/sampling location分类
医药卫生引用本文复制引用
邱珊芳,丁小强,吴薇薇,李阳,方艺,焦晓燕,邵文琦,赵栓,滕杰,蔡洁茹..基于广义估计方程评估尿液混匀程度对慢性肾脏病患者24 h尿蛋白定量的影响[J].中国临床医学,2024,31(3):428-432,5.基金项目
国家自然科学基金(82270719),福建省卫生健康科技计划项目(2022QNB019),福建省2023年省级临床重点专科建设项目,上海市肾脏疾病与血液净化重点实验室(20DZ2271600),上海市肾脏疾病临床医学研究中心(22MC1940100).Supported by National Natural Science Foundation of China(82270719),Health Science and Technology Program of Fujian Health Commission(2022QNB019),Fujian Provincial Key Clinical Specialty in 2023,Shanghai Key Laboratory of Kidney and Blood Purification(20DZ2271600),Shanghai Clinical Research Center for Kidney Disease(22MC1940100). （82270719）
