中国司法鉴定Issue(3)：55-59,5.DOI:10.3969/j.issn.1671-2072.2024.03.008
高效液相色谱-串联质谱法测定电子烟油中的替来他明和依托咪酯
Determination of Tiletamine and Etomidate in E-Cigarette Oil by HPLC-MS/MS
摘要
Abstract
Objective To establish a rapid method for the determination of tiletamine and etomidate in e-cigarette oil using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Methods The samples were diluted with methanol and filtered through filter membrane.C18 chromatography column(2.1 mm×100 mm,2 μm)was used as separation column.Gradient elution was performed with the mobile phase of acetonitrile-0.1%(volume fraction)formic acid aqueous solution.The multiple reaction monitoring(MRM)mode and positive ionization was used for the qualitative and quantitative analysis of tiletamine and etomidate.Results Tiletamine had good linear relationship in the concentration range of 0.02-10.00 ng/mL,and etomidate had good linear relationship in the concentration range of 0.05-10.00 ng/mL.The correlation coefficients were greater than 0.99.The limit of detection for tiletamine and etomidate were 0.01 ng/mL and 0.02 ng/mL,respectively,and the limit of quantification were 0.02 ng/mL and 0.05 ng/mL,respectively.In the spike recovery experiment,the recovery rate of the samples ranged from 95.81%to 102.05%.Conclusion The method is simple to operate with good reproducibility and can be applied to the qualitative and quantitative detection of trace amounts of tiletamine and etomidate in e-cigarette oil.关键词
高效液相色谱-串联质谱法/替来他明/依托咪酯/电子烟油Key words
high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)/tiletamine/etomidate/e-cigarette oil分类
社会科学引用本文复制引用
马俊,闫昕,张腾,雷佳眉,李楠,崔哲,刘兴亮,赵霞..高效液相色谱-串联质谱法测定电子烟油中的替来他明和依托咪酯[J].中国司法鉴定,2024,(3):55-59,5.基金项目
北京市公安局技术研究科研项目(2023CX23012). （2023CX23012）
