中国烟草科学2024，Vol.45Issue(3)：51-59,67,10.DOI:10.13496/j.issn.1007-5119.2024.03.008
湖南烟草镰刀菌根腐病病原鉴定及防治药剂筛选
Pathogen Identification of Tobacco Root Rot Disease in Hunan Province and Screening of Control Fungicides
摘要
Abstract
Aiming to identify the pathogen types of tobacco root rot disease in Chenzhou and Yongzhou regions of Hunan Province,the effective control agents were screened for the main pathogens,samples of tobacco root rot disease and rhizosphere soil were collected from the main tobacco-growing area and isolated by using tissue separation method and plate dilution method,respectively.The pathogen types were determined by morphological observation,EF-1α sequence phylogenetic analysis,and pathogenicity assays.The growth rate method was employed to determine inhibitory effect of the tested fungicides on mycelia growth of the main pathogenic Fusarium species.The results showed that 128 fungal strains was isolated,including 87 Fusarium strains,which were divided into Fusarium commune,Fusarium incarnatum,Fusarium fujikuroi,Fusarium solani and Fusarium proliferatum,Fusarium falciforme,Fusarium humulium.Among them,F.commune accounted for the highest proportion of 39.08%,followed by F.solani and F.incarnatum,both with the proportions of 14.94%.Virulence tests showed that these Fusarium strains could cause tobacco root rot disease and were identified as the pathogen of this disease.F.commune was sensitive to 40%Prochloraz EW,43g/L Tebuconazole SC and 15%cyanomethacrylate SC with EC50 of 0.026,0.079 μg/mL and 0.169 μg/mL,respectively.The results of pot experiment indicated that 40%Prochloraz EW exhibited the most significant control effect,with relative control effect of 94.43%±2.79%,followed by 43 g/L Tebuconazole SC and 15%Phenamacril SC,with the control efficiency of 88.63%±1.68%and 83.07%±2.55%,respectively.This study demonstrates that Fusarium commune is the main pathogen of tobacco root rot disease in Chenzhou and Yongzhou of Hunan Province.Among the tested agents,40%Prochloraz EW proves the best control effect on this pathogen.关键词
烟草根腐病/镰刀菌/致病性/共享镰刀菌/药剂筛选Key words
tobacco root rot/Fusarium/pathogenicity/Fusarium commune/fungicides screening分类
农业科技引用本文复制引用
钟杰,肖艳松,邱泽澜,刘仕达,周向平,王迎,黄焱斌,周可心,朱俊子,刘天波..湖南烟草镰刀菌根腐病病原鉴定及防治药剂筛选[J].中国烟草科学,2024,45(3):51-59,67,10.基金项目
湖南省烟草公司郴州市公司烟草农业科技创新项目(CZYC2022JS03、CZYC2023JS05、CZYC2024JS05) （CZYC2022JS03、CZYC2023JS05、CZYC2024JS05）
中国烟草总公司湖南省公司科技重点项目(HN2021KJ01) （HN2021KJ01）
