中国烟草科学2024，Vol.45Issue(3)：86-92,7.DOI:10.13496/j.issn.1007-5119.2024.03.012
NtMYB17过表达对烟草腺毛发育的影响
Effect of NtMYB17 Overexpression on Development of Glandular Trichomes in Tobacco
摘要
Abstract
As the front line of biological and abiotic defense,and the site of secretion and synthesis of secondary metabolites,regulation of tobacco glandular hair is of great importance for tobacco stress resistance and quality breeding.To explore the function of MYB transcript factors in tobacco glandular trichomes development,one MYB transcription factor NtMYB17 gene were cloned from common tobacco in this study.The analysis involved examining the conserved domain,assessing tissue-specific gene expression via qRT-PCR,and conducting a subcellular localization to further scrutinize the expression pattern of the gene.Results showed that NtMYB17 had a total length of 939 bp,encoding 312 amino acids,a typical MYB domain and was localized in the nucleus at the subcellular level.qRT-PCR revealed that the expression of NtMYB17 in glandular trichomes was eight times higher than that in trichome-free leaves.The expression level was higher in flower buds and young leaves,and it was lowest in roots.Following the overexpression of NtMYB17 in tobacco,an increase was observed in the number of branch-type glandular trichomes.This study indicated that NtMYB17played a regulatory role in the morphology of tobacco glandular trichomes.关键词
烟草/腺毛/MYB转录因子/过表达Key words
tobacco/glandular trichomes/MYB transcription factor/overexpression分类
农业科技引用本文复制引用
张哲,龚达平,王东,何鑫玺,张纪利,韦建玉,黄崇峻,田震,孙晋浩,李志远,陈明丽..NtMYB17过表达对烟草腺毛发育的影响[J].中国烟草科学,2024,45(3):86-92,7.基金项目
中央级公益性科研院所基本科研业务费专项(1610232023013) （1610232023013）
湖南中烟工业有限责任公司科技项目(KY2020YC0008) （KY2020YC0008）
中国烟草总公司山东省公司科技项目(202011) （202011）
中国烟草总公司重大科技项目[110202201014(JY-14)] （JY-14）
