广东海洋大学学报2024，Vol.44Issue(4)：27-37,11.DOI:10.3969/j.issn.1673-9159.2024.04.004
虾青素对赤点石斑鱼生长、体色、皮肤色素和抗氧化性的影响
Effects of Astaxanthin on Growth,Body Color,Skin Pigment,and Antioxidant Capacity of Epinephelus akaara
摘要
Abstract
[Objective]To explore the effects of astaxanthin on growth,body color,skin pigment,and antioxidant activity of red-spotted grouper(Epinephelus akaara),and to provide scientific basis for the application of astaxanthin in the feed of red-spotted grouper.[Method]0,100,200,400 and 800 mg/kg astaxanthin were added to the basic diets of red-spotted grouper,and 5 kinds of iso-nitrogen and iso-lipid diets were prepared,which were recorded as the group AST0(control),AST100,AST200,AST400 and AST800,respectively.The fish with initial body mass(BM)of(6.17±0.26)g and total length of(7.84±0.54)cm were fed on the diets for 90 days.The growth performance indexes of the grouper were measured.The body color parameters of the grouper were measured by colorimeter.The concentrations of carotenoids and melanin in the skin of the grouper were determined by one-step sandwich technique enzyme linked immunosorbent assay(ELISA).The total antioxidant capacity(T-AOC)of liver and other antioxidant properties of the grouper were measured by a kit.[Result]Compared with AST0 group,there were no significant differences in body mass(BM),weight gain rate(WGR),specific growth rate(SGR),survival rate,feed conversion ratio and condition factor in all experimental groups(P>0.05),while hepatosomatic index in AST800 group was significantly decreased(P<0.05).With the increase of astaxanthin in the diet,the skin spots on the head,side and tail of the fish become orange-yellow,and the base of the ventral fins become dark red.The density of pigmented cells in dorsal fin,anal fin and tail fin was high,while the density of pigmented cells in pectoral fin and ventral fin was low.Adding 100 mg/kg or more of astaxanthin in the diet can increase the number of xanthophores and erythrophores in the ventral fins of red-spotted grouper.The brightness(L*)of operculum skin,dorsal skin,caudal peduncle skin,pectoral fin and dorsal fin of AST100,AST200,AST400 and AST800 groups were significantly higher than that of AST0(P<0.05),the redness(a*)of operculum skin and dorsal fin was significantly higher than that of AST0,and the yellowness(b*)of operculum skin,dorsal skin,ventral skin,caudal peduncle skin,pectoral fin,dorsal fin and caudal fin were significantly increased compared with AST0(P<0.05).Dietary astaxanthin significantly increased the carotenoid concentration in the back and ventral skin and the melanin concentration in the ventral skin(P<0.05).The activities of T-AOC,catalase,superoxide dismutase and glutathione peroxidase in liver of red-spotted grouper were significantly increased with the increase of dietary astaxanthin supplemental level(P<0.05),and the content of malondialdehyde was significantly decreased with the increase of dietary astaxanthin supplemental level(P<0.05).[Conclusion]Dietary astaxanthin had no significant effects on BM,WGR and SGR,but significantly improved body color and internal pigmentation,and increased liver antioxidant capacity.Based on the experimental data of growth,body color,skin pigment content and antioxidant activity,the optimal supplemental level of astaxanthin in the diet of red-spotted grouper is 200-400 mg/kg.关键词
赤点石斑鱼/虾青素/体色/皮肤色素/抗氧化性Key words
Epinephelus akaara/astaxanthin/body color/skin pigment/antioxidant capacity分类
农业科技引用本文复制引用
苏金枝,刘阳,王清滨,赵侠,孙芳芳,田永胜,李胜忠,邓远,李振通,黎琳琳,邱弈树,丁小雨,段鹏飞,王心怡,王林娜..虾青素对赤点石斑鱼生长、体色、皮肤色素和抗氧化性的影响[J].广东海洋大学学报,2024,44(4):27-37,11.基金项目
国家重点研发计划(2022YFD2400502) （2022YFD2400502）
泰山产业领军人才项目(LJNY202109) （LJNY202109）
山东省重点研发项目(2022LZGC016) （2022LZGC016）
海南省重点研发项目(ZDYF2022XDNY243) （ZDYF2022XDNY243）
青岛市自然科学基金(23-2-1-53-zyyd-jch) （23-2-1-53-zyyd-jch）
财政部和农业农村部:国家现代农业海水鱼产业技术体系专项资助项目(CARS-47-G31) （CARS-47-G31）
中央公益性科学研究所基础研究基金(2020TD19) （2020TD19）
黄海水产研究所科研经费(20603022021010) （20603022021010）
青岛博士后应用研究项目 （）
