广东海洋大学学报2024，Vol.44Issue(4)：38-46,9.DOI:10.3969/j.issn.1673-9159.2024.04.005
耐盐好氧反硝化菌黏质沙雷氏菌HL4的分离鉴定及脱氮性能
Characterization and Nitrogen Removal Performance of a Salt-tolerant Aerobic Denitrifying Bacterium Serratia marcescens HL4
摘要
Abstract
[Objective]To isolate and identify an efficient salt tolerant aerobic denitrifying bacterial strain and strengthen the research of denitrization technology in seawater and brackish water culture.[Method]Using the in-situ sediment of Lateolabrax maculatus pond as screening objects,a strain HL4 with the best denitrification function was initially isolated by dilution coating method.The strain was identified by 16s rDNA sequencing and physiological and biochemical experiments.The optimal culture conditions and denitrifying performance were investigated by setting different carbon sources,carbon nitrogen mass ratio(mC/mN),pH,temperature,salinity and other conditions.The environmental safety of the strain was tested by challenge test(with healthy zebrafish,Danio rerio,as the test organism).[Result]The strain HL4 was identified as Serratia marcescens,named S.marcescens HL4.Using glucose or sodium succinate as carbon source,the strain HL4 had strong nitrogen removal ability at mC/mN=20,pH=6.0-7.0,and temperature 30℃,48 h TN removal rate was 81.83%-87.17%.The strain could grow in 0-30 salinity environment and had a certain nitrogen removal ability.The 72 h TN removal rate at 0-10 salinity was 68.76%-76.59%,and the 72 h TN removal rate at 20-30 salinity was 23.84%-53.94%.When the concentration of NH4+-N was 0-100 mg·L-1,the 72 h removal rate of NH4+-N was above 90%;when the concentration of NH4+-N was 500 mg·L-1,the 72 h removal rate of NH4+-N is as high as 73.52%.After 15 days in water containing 1×106 CFU·mL-1 strain HL4,the survival rate of healthy zebrafish was 100%.[Conclusion]Strain HL4 has high biosafety and environmental safety,and has potential application value in the treatment of high-concentration ammonium wastewater,brackish water and low-salinity aquaculture tail water.关键词
黏质沙雷氏菌/好氧反硝化/耐盐/生物安全性/环境因子/脱氮能力Key words
Serratia marcestica/aerobic denitrification/salt-tolerance/biosafety/environmental factors/nitrogen removal capacity分类
农业科技引用本文复制引用
刘凤坤,黄文,赵吉臣,许敬轩,郑雅之,鲁慧杰,孙育平,阮灼豪,郭慧,杨俊贤..耐盐好氧反硝化菌黏质沙雷氏菌HL4的分离鉴定及脱氮性能[J].广东海洋大学学报,2024,44(4):38-46,9.基金项目
广东省农业科学院人才项目(R2020YJ-QG001,R2022PY-QY008) （R2020YJ-QG001,R2022PY-QY008）
珠海市斗门区白蕉镇政府服务项目(ZHWZ2021-403FW,ZHWZ2021-408FW) （ZHWZ2021-403FW,ZHWZ2021-408FW）
乡村振兴战略专项资金(农业科技能力提升)项目(2023工作站12) （农业科技能力提升）
广东省农业科学院协同创新中心课题(202137,202138,XT202231) （202137,202138,XT202231）
