广东海洋大学学报2024，Vol.44Issue(4)：96-104,9.DOI:10.3969/j.issn.1673-9159.2024.04.012
罗非鱼优势耐冰温假单胞菌的分离鉴定及耐冰温基因
Isolation and Identification of Dominant Pseudomonas spp.from Tilapia under Super-chilled Storage and Ice-tolerant Genes of Pseudomonas Strains
摘要
Abstract
[Objective]The aim of this study is to analyze the unique genetic characteristics and ice-tolerant genes of dominant ice-tolerant Pseudomonas strains isolated from tilapia.[Methods]The dominant Pseudomonas strains were first identified using full-length 16S rDNA sequencing from tilapia under super-chilled storage.These strains were then isolated,identified,and subjected to de novo genome sequencing using the Illumina platform.Gene family analysis was performed to determine the core gene families of these ice-tolerant Pseudomonas strains,which were subsequently compared to the genome of the ice-intolerant Pseudomonas aeruginosa PAO1 to identify the unique core gene families of these and then screened out the potential ice-tolerant genes in combination with the cold-adaptive genes of Pseudomonas reported in the literature.[Result and conclusion]Five strains of spoilage bacteria with the highest abundance in tilapia under super-chilled storage were discovered and isolated,and they were identified as Pseudomonas bubulae and Pseudomonas fragi.These five Pseudomonas strains shared 3 692 core gene families,of which 861 were absent in the genome of ice-intolerant P.aeruginosa.These 861 unique core gene families were significantly enriched in pathways related to quorum sensing,steroid degradation,metabolism of certain amino acids and peptides,fatty acid degradation,ascorbate and aldarate metabolism,limonene and pinene degradation,butanoate metabolism,styrene degradation,geraniol degradation and others.Further screening revealed that these five ice-tolerant Pseudomonas strains possessed the potential ice-tolerant genes,such as des,cbpM,ousA,treP,treR,trxC,and grX,which were absent in the genome of ice-intolerant P.aeruginosa.关键词
罗非鱼/冰温贮藏/假单胞菌/鉴定/基因组测序/耐冰温基因Key words
Tilapia/super-chilled storage/Pseudomonas/identification/genome sequencing/ice-tolerant gene分类
轻工纺织引用本文复制引用
陈惠萍,梁桉婕,吕冉晖,陈鸿,卢盛佳,薛佳,缪小兰,段杉..罗非鱼优势耐冰温假单胞菌的分离鉴定及耐冰温基因[J].广东海洋大学学报,2024,44(4):96-104,9.基金项目
广东省自然科学基金(2021A1515010590) （2021A1515010590）
