安徽农业大学学报2024，Vol.51Issue(3)：434-440,7.DOI:10.13610/j.cnki.1672-352x.20240710.021
中华蜜蜂泛素基因及其全长转录本的发掘及分析
Exploration and investigation of Apis cerana cerana ubiquitin genes and their full-length transcripts
摘要
Abstract
This study aimed to explore ubiquitin genes and their full-length transcripts in Apis cerana cerana using three-generation Nanopore sequencing technology.Based on the previously obtained full-length transcrip-tome data of the intestinal tract of 4-6-day-old worker larvae of Apis cerana cerana,the sequences of full-length transcripts were aligned to KEGG and Nr databases using BLAST tools to identify ubiquitin genes and their full-length transcripts.Astalavista software was used to analyze alternative splicing(AS)events of ubiquitin genes and verified by RT-PCR.Additionally,the TAPIS pipeline software was employed to analyze alternative polyad-enylation(APA)sites of ubiquitin genes.A total of 48 ubiquitin genes of Apis cerana cerana and 435 ubiquitin gene related full-length transcripts were identified.A total of 74 AS events of 48 ubiquitin genes were identified,including 31 intron retention events,19 variable 3′-end splicing events,16 variable 5′-end splicing events and 8 exon mutual exclusion events.RT-PCR results confirmed that the three randomly selected AS event types were real and reliable.Furthermore,38 ubiquitin genes were forecasted to contain one or more of the APA site,and in the upstream of the APA site identification to multiple motifs,consistency sequence was:KCWYTDYTMWSYG MWSCARAWCCAG AATGAYCCWYWGGHWVMWGWDRTRGC.The results of this study enrich the data-base of ubiquitin genes and their full-length transcripts information of Apis cerana cerana,and provide resource and foundation for further research on related functions.关键词
东方蜜蜂/中华蜜蜂/全长转录本/泛素/可变剪接/可变多聚腺苷酸化Key words
Apis cerana/Apis cerana cerana/full-length transcript/ubiquitin/alternative splicing/alterna-tive polyadenylation分类
农业科技引用本文复制引用
刘小玉,张佳欣,高旭泽,冯佩林,蔡宗兵,那志豪,陈大福,郭睿,徐国钧..中华蜜蜂泛素基因及其全长转录本的发掘及分析[J].安徽农业大学学报,2024,51(3):434-440,7.基金项目
国家自然科学基金项目(32172792,31702190),国家现代农业产业技术体系专项资金项目(CARS-44-KXJ7),福建省自然科学基金面上项目(2022J01131334),福建农林大学硕士生导师团队项目(郭睿),福建农林大学科技创新专项基金项目(郭睿),宁夏回族自治区重点研发项目(2022BBF02037)和福建农林大学动物科学学院(蜂学学院)科研扶持项目(郭睿)共同资助. （32172792,31702190）
