安徽农业科学2024，Vol.52Issue(13)：157-162,205,7.DOI:10.3969/j.issn.0517-6611.2024.13.039
气相色谱-串联质谱法快速定量测定蔬果及食用菌中126种农药及代谢物残留量
Rapid Quantitative Determination of 126 Pesticides and Metabolites Residues in Vegetables, Fruits and Edible Fungi by Gas Chro-matography-tandem Mass Spectrometry Method
唐淑军 1周春梅 1梁幸 1陈怡 1宋晓仪 1宋世文 1张玲1
作者信息
- 1. 深圳市质量安全检验检测研究院,广东深圳 518101
- 折叠
摘要
Abstract
[Objective]A gas chromatography-tandem mass spectrometry method was established for the rapid quantitative determination of 126 pesticide residues in vegetables, fruits and edible fungi.[Method] After homogenizing the sample, 10.0 g of the sample was weighed and 10.0 mL of acetonitrile was added.The sample was frozen for 10 minutes, and the extraction salt was added for high-speed oscillation extrac-tion for 3 minutes.The extraction solution was purified by dispersed solid-phase extraction and centrifuged.The supernatant was taken and measured by gas chromatography-tandem mass spectrometry on a microporous filter membrane.The matrix matching standard external standard method was used for quantification.[Result] The linear range of this method was 0.005-0.500 mg/L, with correlation coefficient of 0.9912-0.9997;the recovery rates of vegetables, fruits and edible mushrooms were 81.1％-107.2％ with four spiked concentrations of quantification limit, low, medium and high, and the RSD (n=6) was 1.0％-12.3％.[Conclusion] The pre-treatment method of this method saves detection time and solvents, and selects high-performance and long-life chromatography columns suitable for pesticide residue detection (the number of detection samples is three times that of general chromatography columns), greatly saving costs and improving detection effi-ciency.The method is accurate, fast, simple, environmentally friendly, and economical, meeting the testing needs of large quantities of actual samples.关键词
气相色谱-串联质谱法/快速定量测定/蔬果/食用菌/农药残留Key words
Gas chromatography-tandem mass spectrometry method/Rapid quantitative determination/Vegetables and fruits/Edible fungi/Pesticides residues分类
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唐淑军,周春梅,梁幸,陈怡,宋晓仪,宋世文,张玲..气相色谱-串联质谱法快速定量测定蔬果及食用菌中126种农药及代谢物残留量[J].安徽农业科学,2024,52(13):157-162,205,7.
