安徽医科大学学报2024，Vol.59Issue(5)：747-752,760,7.DOI:10.19405/j.cnki.issn1000-1492.2024.05.001
顺铂致急性肾损伤肾脏糖酵解和氨基酸代谢改变的研究
Changes in renal cell glycolysis and amino acid metabolism during cisplatin-induced acute kidney injury
摘要
Abstract
Objective To evaluate the change of energy metabolism during cisplatin-induced acute kidney injury.Methods Adult CD-1 male mice were intraperitoneally injected with a single dose of cisplatin (20 mg/kg), and renal function and renal tissue pathology were tested;gene expression was analyzed and signaling pathways were en-riched in cisplatin-treated renal tubular epithelial cells using transcriptome; the contents of renal glycolysis and a-mino acid metabolites were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) .Re-sults Serum urea nitrogen and blood creatinine significantly increased in cisplatin-treated mice.Pathological his-tology observed swelling and shedding of renal tubular epithelial cells.Transcriptome analysis revealed that 2632 genes were upregulated and 2799 genes were downregulated in cisplatin-treated HK-2 cells.GO and KEGG analy-sis showed that differential genes were enriched in energy metabolism.The GSEA analysis results showed that cispl-atin caused an upregulation of the oxidative phosphorylation pathway and a downregulation of the glycolysis pathway in renal tubular epithelial cells, further KEGG analysis demonstrated that cisplatin caused changes in the expression of amino acid genes in renal cells.Metabolomics showed that the contents of glycolytic intermediates and several a-mino acids were altered in the kidney of cisplatin-treated mice.Conclusion Cisplatin-induced acute renal injury is accompanied by modification in renal tubular cell glycolysis and amino acid metabolism.关键词
顺铂/急性肾损伤/细胞代谢/糖酵解/氨基酸代谢Key words
cisplatin/acute kidney injury/cellular metabolism/glycolysis/amino acid metabolism分类
医药卫生引用本文复制引用
徐申,梁楠楠,任亚辉,何奕樟,张涛,于德新..顺铂致急性肾损伤肾脏糖酵解和氨基酸代谢改变的研究[J].安徽医科大学学报,2024,59(5):747-752,760,7.基金项目
国家自然科学基金(编号:82204493) （编号:82204493）
安徽省自然科学基金(编号:2208085QH237) （编号:2208085QH237）
