安徽医科大学学报2024，Vol.59Issue(5)：834-839,6.DOI:10.19405/j.cnki.issn1000-1492.2024.05.014
脂肪量和肥胖相关蛋白FTO调控IFIT2促进肝细胞癌发生发展
FTO promotes the development of hepatocellular carcinoma via regulating IFIT2
摘要
Abstract
Objective To study the molecular mechanism of fat mass and obesity-associated protein (FTO) regula-ting hepatocellular carcinoma (HCC).Methods HepG2 cells of knock-down FTO were constructed, HepG2 cells of knock-down FTO and HepG2 cells were collected, and high-throughput sequencing was performed using Illumina Hiseq platform to screen the gene expression differences between the two groups.Through GO and KEGG enrich-ment analysis of these differential genes, FTO regulatory pathways were studied and downstream target genes of FTO were screened.The role of FTO downstream target gene in HCC was revealed by bioinformatic analysis and cell ex-periments.Results Transcriptome sequencing showed that386 genes were differentially expressed between HepG2 cells of knock-down FTO and HepG2 cells, and they were involved in biological processes such as response to inter-feron-gamma.The expression of IFIT2, one of the most responsive interferon-stimulating genes, was up-regulated after FTO knockdown.Potential m6 A methylation occurred at multiple sites of IFIT2.The survival of HCC patients with high expression of IFIT2 was significantly prolonged, and knock-down of IFIT2 promoted the growth and migra-tion of HepG2 cells.Conclusion FTO may regulate IFIT2 by mediating m6A, and further promote the occurrence and development of HCC.关键词
FTO/IFIT2/肝细胞癌/转录组测序/HepG2/m6AKey words
FTO/IFIT2/hepatocellular carcinoma/transcriptome sequencing/HepG2/m6A分类
医药卫生引用本文复制引用
兰兰,宣自学,姜金颖..脂肪量和肥胖相关蛋白FTO调控IFIT2促进肝细胞癌发生发展[J].安徽医科大学学报,2024,59(5):834-839,6.基金项目
浙江省公益技术应用研究计划项目(编号:LYY21H-310008) （编号:LYY21H-310008）
浙江省医药卫生科技计划项目(编号:2022KY060、2023KY459、2023KY524) （编号:2022KY060、2023KY459、2023KY524）
