安徽医科大学学报2024，Vol.59Issue(5)：840-846,7.DOI:10.19405/j.cnki.issn1000-1492.2024.05.015
胶束莪术醇对卵巢癌相关巨噬细胞极化的影响
Effect of micellar curcumol on polarization of macrophages associated with ovarian cancer
摘要
Abstract
Objective To investigate the mechanism of micellar curcumol (MC) regulating the immune microenvi-ronment of ovarian cancer by promoting the polarization of M2-type macrophages to M1-type in ovarian cancer asci-tes.Methods ① After the mice were divided into groups, a mouse ovarian cancer ascites model was constructed by using the mouse ovarian cancer cell line ID8.Then weight changes were observed, tumor tissue and ascites were collected.The expression of CD86 and CD206 on macrophages of the tumor tissue and ascites was detected by flow cytometry.The expression of protein kinase B (PKB/Akt)/mammalian target of rapamycin (mTOR) was detected by Western blot.②A human monocytic leukemia cell line (THP-1) was induced to transform into M2 macrophage (THP-1 M2 macrophage) in vitro, and then treated with 10μg/ml MC.The apoptosis was detected by flow cytom-etry.The mRNA levels of macrophage mannose receptor (CD206), transforming growth factor-β(TGF-β), inter-leukin (IL)-1β and tumor necrosis factor-α (TNF-α) were detected by qRT-PCR.The expression of CD86 and CD206 was detected by flow cytometry, and Akt/mTOR expression and phosphorylation was detected by Western blot.Results ① In vitro study showed that the average body weight of the MC group was lower than that of the control group.Compared with the control group, CD206 expression of macrophages decreased in tumor tissue and ascites in the MC group, while the expression of CD86 increased.The Akt and mTOR phosphorylation level of mac-rophages in the MC group's ascites was lower than that in control group.②In vivo study showed that there was no difference in apoptosis rate among the groups detected by flow cytometry.The mRNA expression level of CD206, TGF-β and the protein expression level of CD206 in MC group were significantly lower than those in the control group, while the mRNA expression of IL-1β, TNF-α and the protein expression level of CD86 were significantly higher than those in the control group.Compared with the control group, the phosphorylation level of Akt and mTOR in the MC group decreased.Conclusion MC promotes M1 polarization of macrophages in ascites to regulate the immune microenvironment of ovarian cancer, which may be related to the Akt/mTOR pathway.关键词
卵巢癌/免疫微环境/巨噬细胞/MC/Akt/mTORKey words
ovarian cancer/immune microenvironment/macrophages/MC/Akt/mTOR分类
医药卫生引用本文复制引用
唐勤,王晶,陈冰,汪生,张敏敏,张梦媛,吴强..胶束莪术醇对卵巢癌相关巨噬细胞极化的影响[J].安徽医科大学学报,2024,59(5):840-846,7.基金项目
国家自然科学基金(编号:82104633) （编号:82104633）
安徽高校研究生科学研究项目(编号:YJS20210282) （编号:YJS20210282）
安徽高校自然科学研究项目(编号:2023AH053293、KJ2020A0200) （编号:2023AH053293、KJ2020A0200）
