安徽医科大学学报2024，Vol.59Issue(6)：983-988,6.DOI:10.19405/j.cnki.issn1000-1492.2024.06.011
补充褪黑素的人卵母细胞体外培养成熟技术在COH周期中的应用
Application of melatonin-supplemented in vitro maturation technology for human oocytes during COH cycle
摘要
Abstract
Objective To compare the early embryonic developmental potential and clinical outcomes of oocytes matured in vivo and those matured by modified in vitro maturation(LVM)technology during the same controlled ovarian hyperstimulation(COH)cycle,and to explore the clinical application of melatonin-supplemented IVM technology.Methods 159 patients were recruited into the study.920 mature oocytes were collected during their COH cycles processed for conventional IVF/ICSI protocols,while 1 283 immature oocytes from the same cycles were matured in a melatonin-supplemented IVM medium before ICSI was performed.A retrospective analysis was conducted to compare the impact of conventional assisted reproductive technology and improved IVM technology on the outcomes of assisted reproductive therapy and pregnancy outcomes.Results Compared with mature oocytes collected from COH cycles treated with conventional IVF/ICSI,oocytes promoted by improved melatonin-supple-mented IVM technology had a lower rate of high-quality blastocyst formation.However,after embryo transfer,there was no significant difference in the clinical outcomes of mature oocytes obtained through two methods,including clinical pregnancy rate,full-term birth rate,neonatal length,and neonatal Apgar score.Conclusion The applica-tion of melatonin-supplemented IVM significantly increases the utilization of immature oocytes collected from COH cycles,improving the pregnancy outcomes of patients assisted by assisted reproductive technology.关键词
卵母细胞/体外培养成熟技术/体外受精/控制性超促排卵/妊娠结局Key words
oocyte/in vitro maturation technology/in vitro fertilization/controlled ovarian hyperstimulation/pregnancy outcome分类
医药卫生引用本文复制引用
任宇,韩星星,张琦琦,刘璐,许孝凤,章志国,邹慧娟..补充褪黑素的人卵母细胞体外培养成熟技术在COH周期中的应用[J].安徽医科大学学报,2024,59(6):983-988,6.基金项目
国家重点研发计划资助项目(编号:2022YFC2703000) （编号:2022YFC2703000）
国家自然科学基金(编号:32000642、82071724) （编号:32000642、82071724）
安徽省卫健委重点项目(编号:AHWJ2023A10019) （编号:AHWJ2023A10019）
安徽省重点研发与开发计划项目(编号:202204295107020041) （编号:202204295107020041）
安徽医科大学校科研基金(编号:2023xkj158) （编号:2023xkj158）
