安徽医科大学学报2024，Vol.59Issue(6)：1013-1022,10.DOI:10.19405/j.cnki.issn1000-1492.2024.06.016
Cuproptosis基因和特征基因预测急性髓系白血病预后、免疫和肿瘤微环境的价值
Value of Cuproptosis genes and characteristic genes in predicting prognosis,immunity and tumor microenvironment in acute myeloid leukemia
摘要
Abstract
Objective To screen Cuproptosis genes and characteristic genes for differential prognosis in acute mye-loid leukemia(AML)and explore their prognosis in AML as well as their biological roles and correlations in the immune and tumor microenvironment.Methods AML clinical,transcriptome,genomic,and copy number data were downloaded from three major databases,TCGA,GEO,and UCSC,and Cuproptosis genes were collected from published studies.From the perspective of multiomics,the effects of Cuproptosis gene and characteristic gene on survival,immunity,tumor microenvironment,stem cell correlation and drug sensitivity were studied by various bioinformatics methods,meta-analysis and secondary typing.Results One Cuproptosis gene was identified as a differential prognostic gene in AML and five characteristic genes were identified as influencing the prognosis of AML patients by influencing Cuproptosis,and a prognostic model was established.The differential genes were mainly concentrated in mitochondrial activity,REDOX enzyme and energy metabolism.In terms of immunity,macrophage M0,neutrophils,activated memory CD4 T cells and Tregs were positively correlated with risk score,while macro-phage M2,resting mast cells,immature CD4 T cells,helper follicular T cells and memory B cells were negatively correlated with risk score.In terms of tumor microenvironment,the immune cell score of the low-risk group was lower than that of the high-risk group,and in the total score,the tumor microenvironment score of the low-risk group was also lower than that of the high-risk group,indicating that the tumor purity of the high-risk group was lower than that of the low-risk group.However,there was no significant association between stem cells in the high-risk and low-risk groups,and a total of 14 drugs were found to be sensitive to treat AML.Conclusion Cuproptosis gene and characteristic gene are closely related to immune and tumor microenvironment in AML by constructing a prognostic model of AML.关键词
Cuproptosis/急性髓系白血病/差异基因/免疫浸润/肿瘤微环境Key words
Cuproptosis/acute myeloid leukemia/differential gene/immune infiltration/tumor microenvironment分类
医药卫生引用本文复制引用
李寅臻,农卫霞,张眉,芮东升,雷伟,摆文丽,李瑞,张亚洲,王奎..Cuproptosis基因和特征基因预测急性髓系白血病预后、免疫和肿瘤微环境的价值[J].安徽医科大学学报,2024,59(6):1013-1022,10.基金项目
国家自然科学基金(编号:81860374) （编号:81860374）
