安徽医科大学学报2024，Vol.59Issue(6)：1053-1059,7.DOI:10.19405/j.cnki.issn1000-1492.2024.06.022
蒲公英甾醇对Erastin诱导的软骨细胞铁死亡的保护作用及机制
The protective effect and mechanism of Taraxasterol on Erastin induced ferroptosis in chondrocytes
摘要
Abstract
Objective To investigate the role of Taraxasterol(TAR)on ferroptosis in chondrocytes induced by Erastin.Methods The C28/I2 chondrocyte line was treated with Erastin to construct the ferroptosis model of chon-drocytes in vitro and the experiments were divided into Control,Erastin,TAR,and TAR+Erastin groups.Cell via-bility was detected by the CCK-8 assay.Cytotoxicity was detected by the lactate dehydrogenase(LDH)kit and the Calcein/PI cytokinesis kit.Flow cytometry was used to detect lipid reactive oxygen species(ROS).The intracellular glutathione(GSH)content was detected by GSH kit.Mitochondrial membrane potential was detected by JC-1 stai-ning and RH123 staining.ACSL4 and GPX4 protein expression and the key indicators of ferroptosis were detected by Western blot.Results TAR restored the decreased cell viability of C28/I2 chondrocytes induced by Erastin treatment as well as reduced Erastin-induced cytotoxicity(P＜0.01).Compared with the control group,the level of intracellular lipid ROS increased(P＜0.01)and the content of GSH decreased(P＜0.01)after treatment with Erastin,while TAR could reduce the production of lipid ROS(P＜0.01)and increase the content of GSH(P＜0.01).TAR restored mitochondrial membrane potential in C28/I2 chondrocytes ferroptosis,decreased ACSL4 pro-tein expression(P＜0.01)and increased GPX4 protein expression(P＜0.01).In addition,TAR restored the re-duced cell viability caused by IL-1 β treatment.Conclusion TAR can inhibit Erastin induced ferroptosis in C28/I2 chondrocytes,which may be related to the regulation of ACSL4 and GPX4 protein expression.关键词
蒲公英甾醇/软骨细胞/铁死亡/骨关节炎Key words
taraxasterol/chondrocytes/ferroptosis/osteoarthritis分类
医药卫生引用本文复制引用
周富丽,王浩,朱仁弟,赵英杰,杨雅茹,周仁鹏,胡伟,鲁超..蒲公英甾醇对Erastin诱导的软骨细胞铁死亡的保护作用及机制[J].安徽医科大学学报,2024,59(6):1053-1059,7.基金项目
安徽省自然科学基金(编号:2208085MH215) （编号:2208085MH215）
安徽省中医药传承创新科研项目(编号:2020cczd05) （编号:2020cczd05）
安徽省重点研究与开发计划项目(编号:202204295107020035) （编号:202204295107020035）
