妊娠早期蜕膜甲状腺激素受体信号异常对蜕膜化进程的影响及其与流产发生的关联OA北大核心CSTPCD
The influence of abnormal thyroid hormone receptor signal of decidua on decidualization in early pregnancy and its association with the occurrence of miscarriage
目的 评估自然流产患者蜕膜血管生成情况,并探讨自然流产患者蜕膜中甲状腺激素受体信号的表达改变.方法 收集行人流术的孕妇蜕膜.以自然流产患者作为实验组,以社会因素行人流术的健康孕妇作为对照组,收集两组人群蜕膜组织.采用Western blot方法,检测蜕膜组织中甲状腺激素受体-α(THR-α)和THR-β的核转位情况,同时使用免疫组化法定位蜕膜组织中 THR-α和THR-β的分布.Western blot测定蜕膜组织中Ⅱ型脱碘酶(DIO2)的蛋白表达水平,免疫荧光法定位DIO2在蜕膜组织分布并测定荧光强度.使用免疫组化,以CD34作为内皮标志物测定蜕膜血管密度.Western blot检测血管内皮生长因子受体(VEGFR-1)的蛋白表达水平.结果 对照组与流产组在年龄、妊娠天数、TSH、T3、T4等基本资料中,差异均无统计学意义.West-ern blot结果提示THR-α和THR-β蛋白核转位置下降,同时蜕膜组织免疫组化染色结果表明自然流产患者蜕膜甲状腺激素受体核转位减少(P＜0.05).Western blot结果表明流产组DIO2蛋白表达较对照组下降,提示自然流产孕妇蜕膜中,甲状腺激素代谢受到影响;免疫荧光实验结果显示流产组DIO2的荧光强度较对照组减弱(P＜0.05).免疫组化法CD34标记蜕膜小血管的结果显示,在流产组中,CD34+血管数量下降,差异有统计学差异(P＜0.05).Western blot结果提示流产组和对照组中VEGFR-1无明显变化,差异无统计学意义.结论 蜕膜局部甲状腺激素受体信号异常可能导致蜕膜血管生成减少,从而参与自然流产的发生.
Objective To evaluate the angiogenesis of decidua in spontaneous abortion patients and to investigate the change of thyroid hormone receptor signal expression in decidua in miscarriage patients.Methods The decidua of pregnant women was collected.Patients with miscarriage were taken as the experimental group and healthy preg-nant women who underwent abortion by social factors were taken as the control group.The nuclear translocation of thyroid hormone receptor α(THR-α)and THR-β in decidua tissues was detected by Western blot method,and the distribution of THR-α and TH IR-β in decidua tissues was determined by immunohistochemistry.The expression of Deiodinase 2(DIO2)in decidua tissues was determined by Western blot.The distribution of DIO2 in decidua tissues was determined by immunofluorescence and the fluorescence intensity was measured.The decidual vessel density was determined by immunohistochemistry using CD34 as an endothelial marker.The protein expression of vascular endothelial growth factor receptor(VEGFR-1)was detected by Western blot.Results There was no sig-nificant difference in age,gestation days,TSH,T3,T4 and other basic data between the control group and the a-bortion group.The results of Western blot showed that the expression levels of THR-α and THR-β protein de-creased,and the results of immunohistochemical staining of decidual tissues showed that the nuclear translocation of decidual thyroid hormone receptor in patients with spontaneous abortion decreased(P＜0.05).Western blotting results showed that the expression of DIO2 protein in the abortion group was lower than that in the control group,suggesting that thyroid hormone metabolism in decidua of spontaneous abortion pregnant women was affected.The results of immunofluorescence test showed that the fluorescence intensity of DIO2 in the abortion group was weaker than that in the control group(P＜0.05).Immunohistochemical CD34 labeling of decidual small blood vessels showed that the number of CD34+blood vessels decreased in the abortion group with statistical difference(P＜0.05).Western blot results showed that there was no significant change in VEGFR-1 between the two groups,and the difference between the two groups was not statistically significant.Conclusion The abnormal signal of thyroid hormone receptors in decidua may decrease decidua angiogenesis and participate in spontaneous abortion.
翟沐鑫;李萱;余涛;赵丽;于震;杨媛媛
安徽医科大学第一附属医院妇产科,合肥 230032||国家卫生健康委配子及生殖道异常研究重点实验室,合肥 230032||生殖健康与遗传安徽省重点实验室,合肥 230032
临床医学
流产蜕膜化甲状腺激素受体
miscarriagedecidualizationthyroid hormone receptor
《安徽医科大学学报》 2024 (006)
1076-1081 / 6
安徽省自然科学基金项目(编号:2008085MH243)
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